Título:	Hypocholesterolemic, antiaterogenic, antimutagenic potential and non-toxic in vivo of sorghum (Sorghum bicolor (l.) Moench) flour and its protein hydrolysate.
Autoria:	GAVA, J. C. NETTO, M. C. T. GRANCIERI, M. DUTRA, K. A. ALVES, N. E. G. MORAES, E. A. GOMES, V. M. CHERENE, M. B. ANDRADE, T. U. QUEIROZ, V. A. V. VASCONCELOS, C. M.
Afiliação:	JULIANA COUTO GAVA, UNIVERSIDADE VILA VELHA; MARITZA CERQUEIRA TEIXEIRA NETTO, UNIVERSIDADE VILA VELHA; MARIANA GRANCIERI, UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO; KARYNNA ALCANTARA DUTRA, UNIVERSIDADE VILA VELHA; NATÁLIA ELIZABETH GALDINO ALVES, UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO; ÉRICA AGUIAR MORAES, UNIVERSIDADE FEDERAL DO ESPIRITO SANTO; VALDIRENE MOREIRA GOMES, UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE-DARCY RIBEIRO; MILENA BELLEI CHERENE, UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE-DARCY RIBEIRO; TADEU UGGERE ANDRADE, UNIVERSIDADE VILA VELHA; VALERIA APARECIDA VIEIRA QUEIROZ, CNPMS; CHRISTIANE MILEIB VASCONCELOS, UNIVERSIDADE VILA VELHA.
Ano de publicação:	2025
Referência:	Brazilian Archives of Biology and Technology, v. 68, e25240595, 2025.
Conteúdo:	Sorghum (Sorghum bicolor (L.) Moench) is an alternative to the consumption of conventional cereals, with high technological, nutritional and functional potential. The peptides formed during the enzymatic digestion of their proteins have shown a modulating effect on variables related to chronic noncommunicable diseases, including cardiovascular ones. The aim of this study was to identify and characterize the bioactive compounds of sorghum and its hydrolysate in their toxicity, hypocholesterolemic, antiatherogenic, and antioxidant properties in vivo. For the toxicity test, nematodes Caenorhabditis elegans were used. For the experimental study, wild-type C57BL/6 and LDL-cholesterol receptor knockout (LDL-/-) mice were maintained on a normal or atherogenic diet and received whole sorghum flour or its hydrolysate by intragastric oral gavage (800 mg/kg) for 8 weeks. The protein hydrolysate showed the highest concentration of phenolic compounds and high antioxidant activity; the enzymatic hydrolysis reached 42.5% of the proteins, with low molecular weight peptides, in addition to not showing toxicity at the dose used. The in vivo results demonstrated that the flour and hydrolysate reduced the levels of total and LDL-c cholesterol and the lipid deposition in aorta of animals with atherogenic diet, and increased the antioxidant activity in plasma and liver, in addition to plasmatic HDL-c. The hydrolysate also showed an antimutagenic effect by micronucleus test. Therefore, whole sorghum flour and its hydrolysate are a source of antioxidant nutritional compounds that act against the development of hypercholesterolemia and atherogenesis, in addition to an antimutagenic effect and absence of toxicity.
Thesagro:	Sorgo Farinha
Palavras-chave:	Composto bioativo Toxicidade
Digital Object Identifier:	http://doi.org/10.1590/1678-4324-2025240595
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMS)