Título:	Targeted redesign and optimization of culture media for ethylene glycol biosynthesis in Komagataella phaffii.
Autoria:	PACHECO, T. F. ALMEIDA, J. R. M. de
Afiliação:	THÁLYTA FRAGA PACHECO, UNIVERSIDADE DE BRASÍLIA; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE.
Ano de publicação:	2025
Referência:	Fermentation, v. 11, n. 8, 424, 2025.
Conteúdo:	Tailoring culture media and supplementation strategies to the specific requirements of a target product is essential for enhancing microbial production efficiency. This work addresses an unexplored aspect of K. phaffii cultivation: optimizing culture media for metabolite production from xylose, diverging from the conventional focus on recombinant protein expression and the use of glycerol or methanol as primary substrates. Ethylene glycol biosynthesis in an engineered K. phaffii strain was improved by evaluating media and nutrient supplementation. Among the seven evaluated formulations, FM22 and d’Anjou were the most effective, with inositol and thiamine dichloride playing key roles in enhancing production. Salt concentrations in both media were optimized using Central Composite Design (CCD), reducing complexity while increasing yields. Ethylene glycol production increased by 54% in FM22 and 21% in d’Anjou, accompanied by a threefold and 26% reduction in the total salt content, respectively. The vitamin solution was streamlined from seven to two components, each at half the standard concentration. Trace element solutions were reduced to 25% of the original volume without compromising productivity. These findings underscore the dual benefit of culture medium optimization: improved ethylene glycol yields and simplified formulations, establishing a foundation for the development of more efficient and cost-effective bioprocesses using K. phaffii.
NAL Thesaurus:	Xylose Ethylene glycol Bioprocess engineering
Palavras-chave:	Komagataella phaffii
Digital Object Identifier:	https://doi.org/10.3390/fermentation11080424
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAE)