Título:	Biotechnological potential of sweet sorghum as a substrate in the production of xylanases and cellulases by actinobacteria.
Autoria:	BARTOLOMEU, R. D. de S. JESUS, E. da C. PARRELLA, R. A. da C. MARRIEL, I. E. SIMEONE, M. L. F.
Afiliação:	RENATA DANIELLE DE SOUZA BARTOLOMEU, UNIVERSIDADE FEDERAL DE LAVRAS; EDERSON DA CONCEICAO JESUS, CNPAB; RAFAEL AUGUSTO DA COSTA PARRELLA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; MARIA LUCIA FERREIRA SIMEONE, CNPMS.
Ano de publicação:	2026
Referência:	Microbiology Research, v. 17, n. 2, 31, 2026.
Conteúdo:	Obtaining enzymes through bioconversion depends on a complex relationship between the microorganisms and the biomass used. Here, we evaluate xylanase production by diverse actinobacterial species, cultivated using xylan as the sole carbon source and complex media containing sorghum as the substrate. Fifty-three actinobacteria were tested for xylanase production in a solid medium. Seventeen strains produced xylanase and were tested for their ability to produce xylanase, total cellulases (filter paper activity, FPase), and endoglycanase in submerged culture using a defined liquid medium. The best xylanaseproducing species was Streptomyces capoamus, yielding 24 IU·mL−1 . For FPase, Streptomyces sp. showed the highest yield (1.12 IU·mL−1 ); for endoglycanase, the best producer was Streptomyces ossamyceticus (0.99 IU·mL−1 ). When sweet sorghum was used alone, S. curacoi, S. ossamyceticus, and S. capoamus showed xylanase activities of 4.5 IU·mL−1 , 4.4 IU·mL−1 , and 0.8 IU·mL−1 , respectively. However, FPase activity was not detected under the assay conditions. The results showed that there is an intraspecific difference in xylanase, endoglucanase, and FPase production by actinobacteria, with the species S. curacoi, S. ossamyceticus, and S. capoamus able to use sorghum as a carbon source, demonstrating biotechnological potential.
Thesagro:	Sorgo Enzima Celulase
NAL Thesaurus:	Actinobacteria
Palavras-chave:	Substrato hemicelulósico Xilanase Sorgo sacarino
Digital Object Identifier:	https://doi.org/10.3390/microbiolres17020031
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMS)