Título:	Synthesis of Arapaima gigas growth hormone (ag-GH) in HEK 293 cells: its purification and characterization via in vivo bioassay in dwarf “Little” mice.
Autor:	LIMA, E. R. MELO, J. C. R. BEZERRA, F. M. SUZUKI, M. F. NUNES, A. P. SEVILHANO, T. C. dos A. OLIVEIRA, J. E. GARCEZ, R. TORATI, L. S. MAGALHÃES, G. S. PERONI, C. N. BARTOLINI, P.
Afiliación:	ELIANA ROSA LIMA, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; JENIFFER CRISTINA RIBEIRO MELO, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; FILIPE MENEZES BEZERRA, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; MIRIAM FUSSAE SUZUKI, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; AMANDA PALERMO NUNES, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; THAIS CRISTINA DOS ANJOS SEVILHANO, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; JOÃO EZEQUIEL OLIVEIRA, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; RIVIANE GARCEZ, UNIVERSIDADE DE SÃO PAULO; LUCAS SIMON TORATI, CNPASA; GERALDO SANTANA MAGALHÃES, INSTITUTO BUTANTAN; CIBELE NUNES PERONI, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES; PAOLO BARTOLINI, INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES.
Año:	2026
Referencia:	Molecules, v. 31, 572, 2026.
Descripción:	Arapaima gigas growth hormone (ag-GH) cDNA was previously cloned from A. gigas pituitaries. In this work ag-GH has been synthesized using human embryonic kidney 293 cells (HEK293) transiently transfected with the 3.4-TOPO® vector carrying ag-GH cDNA. The 4th day after transfection, the presence of putative ag-GH was detected via SDS-PAGE and Western blotting in comparison with human GH. Ion exchange purification exhibited a clearly symmetric peak, absent in the control medium. The purified fraction, submitted to high-performance size-exclusion chromatography (HPSEC), SDS-PAGE, and Western blotting, contained an immunoreactive molecule, slightly smaller than hGH as expected. MALDI-TOF-MS determined a high-resolution molecular mass of 21,220 Da versus a theoretical value of 21,150. A phylogenetic analysis positioned ag-GH within basal teleost lineages, consistent with earlier analyses of A. gigas gonadotrophic hormones, reinforcing the structural and functional conservation relevant for its biologic activity. An in vivo bioassay based on the body weight increase of dwarf “little” mice demonstrated a biological activity for ag-GH comparable to that of the international reference preparation of rec-hGH. For two species (H. sapiens and A. gigas) separated by an evolutionary period of >100 million years, such a positive biological correlation is remarkable.
Thesagro:	Pirarucu Peixe
NAL Thesaurus:	Arapaima gigas
DOI:	https://doi.org/10.3390/molecules31030572
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPASA)