Título:	Soil microbiome responses to carbon substrates in an artificial rhizosphere system.
Autor:	FONTANA, J. LEME, M. M. P. MENDES, R.
Afiliación:	JULIANE FONTANA KOBE, EMBRAPA MEIO AMBIENTE MARCIA MARIA PARMA LEME, CENTER FOR NUCLEAR ENERGY IN AGRICULTURE, UNIVERSITY OF SÃO PAULO RODRIGO MENDES, CNPMA.
Año:	2025
Referencia:	In: PLANT MICROBIOME SYMPOSIUM, 6., 2025, Málaga/Antequera. Abstract Book... Bruxelas: European Plant Science Organisation (EPSO), 2025. p. 163. Poster 090.
Descripción:	The rhizosphere is a hotspot of microbial activity driven by root exudation, where subtle changes in carbon inputs can shape microbiome structure and function. To explore these dynamics, we developed an artificial rhizosphere system designed to mimic natural exudation patterns. The system consists of microrhizons connected to a high-precision peristaltic pump and tubing network, enabling delivery of carbon substrates into the soil at flow rates comparable to those of plant root exudates. Using this setup, we conducted a 14-day microcosm experiment where water (control), sucrose, or nicotinic acid was injected into the soil. After the treatment period, we extracted DNA for both 16S rRNA gene amplicon and whole metagenome sequencing. The amplicon data revealed that sucrose addition led to a marked reduction in alpha diversity, while nicotinic acid had a more moderate impact. Ordination analyses (PCoA of Bray-Curtis distances) showed clear separation between treatments, indicating strong effects of carbon inputs on community structure. Taxonomic analysis showed that sucrose strongly enriched copiotrophic taxa such as Bryobacteraceae and Micromonosporaceae, consistent with their rapid response to simple, labile carbon sources. In contrast, nicotinic acid enriched Micromonosporaceae, Acidothermaceae, and Bacillaceae, suggesting selection for microbial groups potentially involved in complex carbon degradation. Metagenomic analysis further revealed functional shifts in microbial traits linked to carbon substrate catabolism, reinforcing the role of substrate quality in shaping microbial functions. Our findings demonstrate that short-term carbon inputs can rapidly reshape soil microbial communities and their functional potential. This artificial rhizosphere platform offers a powerful approach to uncover the underlying mechanisms governing microbiome assembly and functioning in response to specific exudate signals under controlled conditions.
NAL Thesaurus:	Microbiome metagenomics microbial ecology
Palabras clave:	Artificial rhizosphere Carbon cycling Microbiome assembly
Tipo de Material:	Resumo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Resumo em anais de congresso (CNPMA)