Título:	Seed viability of Commiphora leptophloeos (Mart.) J.B. Gillett (Burseraceae) assessed by the Tetrazolium test.
Autoria:	SILVA, J. C. da SILVA, J. de J. GOMES, R. A. CRUZ, C. R. P.
Afiliação:	JAMILLE CARDEAL DA SILVA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; JAILTON DE JESUS SILVA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; RAQUEL ARUJO GOMES, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; CLAUDINÉIA REGINA PELANCANI CRUZ, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA.
Ano de publicação:	2025
Referência:	International Journal of Plant Biology, v. 16, n. 4, 139, 2025.
Conteúdo:	Commiphora leptophloeos, a native Caatinga species with economic and medicinal potential, faces propagation challenges due to seed dormancy and extractive use. The germination test, the official method for seed quality assessment, is time-consuming, whereas the tetrazolium test (TZT) offers a rapid alternative for determining seed viability. This study aimed to establish and validate a TZT protocol for C. leptophloeos seeds. Seeds collected in 2025 were extracted after natural fruit drying and then stored in a cold chamber. The germination test was conducted with seeds without pyrenes at 30 ◦C. For the TZT, a completely randomized design was used in a 6 × 4 factorial scheme (six TZT concentrations × four immersion times), with adjustments in seed preparation and staining procedures. Higher concentrations (0.5% and 0.75%) combined with shorter immersion periods (2 h) provided the best results, especially 0.75% for 2 h, which yielded 89% viability. Very low concentrations combined with short periods resulted in little or no staining. Compared with the germination test (35%), the TZT showed greater sensitivity in detecting viable seeds. We conclude that the TZT is highly efficient for assessing the viability of C. leptophloeos seeds, with optimal responses at 0.5–0.75% TTC and 2–4 h immersion periods, and represents a strategic tool to support the conservation and sustainable use of this species.
Thesagro:	Espécie Nativa Caatinga Semente Planta Medicinal Teste de Tetrazólio
NAL Thesaurus:	Natural resources
Palavras-chave:	Commiphora leptophloeos Bioma Caatinga Imburana-de-espinho Umburana-de-cambão
Digital Object Identifier:	https://doi.org/10.3390/ijpb16040139
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATSA)