Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1185135
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorABREU, V. A. C. de
dc.contributor.authorALVES, R. M.
dc.contributor.authorOLIVEIRA, M. de M.
dc.contributor.authorTRINCA, V.
dc.contributor.authorFALCAO, L. L.
dc.contributor.authorMARCELLINO, L. H.
dc.contributor.authorFIGUEIRA, A.
dc.contributor.authorDOMINGUES, D. S.
dc.contributor.authorVARANI, A. M.
dc.date.accessioned2026-03-06T17:11:05Z-
dc.date.available2026-03-06T17:11:05Z-
dc.date.created2026-03-06
dc.date.issued2026
dc.identifier.citationThe Plant Genome, v. 19, n. 1, e70196, 2026.
dc.identifier.issn1940-3372.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1185135-
dc.descriptionCupuassu (Theobroma grandiflorum) is a fruit tree native to the Brazilian Amazon and increasingly relevant to regional bioeconomies. Its cultivation is severely affected by witches’ broom disease (WBD), caused by Moniliophthora perniciosa. While a chromosome-scale genome of the susceptible genotype C1074 is available, the lack of a resistant reference has limited investigation into the genomic basis of resistance. Here, we present the first chromosome-scale assembly of the WBD-resistant genotype C174 (415.8 Mb) and a comparative analysis with C1074 integrating structural variant detection, gene-duplication profiling, transposable-element (TE) annotation, and time-resolved host–pathogen transcriptomics. C174 exhibits distinctive tandem and dispersed duplications, genotype-specific TE insertions, and coordinated defense-gene expression, together with higher heterozygosity indicative of broader allelic diversity. Genome-wide, TEs show differential expression and spatial proximity to immune loci, suggesting potential regulatory importance. Although C1074 encodes more nucleotide-binding domain leucine-rich repeat receptors (541 vs. 434), most remain transcriptionally inactive, whereas C174 shows sequential activation of pattern-recognition receptors, mitogen-activated protein kinase components, transcription factors, and pathogenesis-related proteins. Within the Chromosome 6 previously identified resistance quantitative trait locus, two duplicated DUF4220/DUF594 genes (where DUF is domain of unknown function) unique to C174—orthologous to a maize gene implicated in fungal response before—are infection-induced and display signatures of episodic positive selection. Together, these results establish a high-quality genomic framework for exploring the molecular architecture of WBD response in T. grandiflorum. The datasets generated here—including the C174 and C1074 reference genomes, immune-related variant catalog, and prioritized defense-gene lists—constitute a comprehensive open resource for evolutionary, functional, and breeding research of Theobroma species.
dc.language.isoeng
dc.rightsopenAccess
dc.titleIntegrative chromosome‐scale genome analysis of cupuassu provides insights into witches' broom disease resistance and expands genomic resources for Theobroma.
dc.typeArtigo de periódico
dc.subject.thesagroCupuaçu
dc.subject.thesagroTheobroma Grandiflorum
dc.subject.thesagroDoença
dc.subject.thesagroDoença de Planta
dc.subject.thesagroVassoura de Bruxa
dc.subject.thesagroResistência
dc.subject.thesagroGenoma
riaa.ainfo.id1185135
riaa.ainfo.lastupdate2026-03-06
dc.identifier.doihttps://doi.org/10.1002/tpg2.70196
dc.contributor.institutionVINICIUS A. C. DE ABREU, UNIVERSIDADE FEDERAL DO PARÁ; RAFAEL MOYSES ALVES, CPATU; MAURO DE MEDEIROS OLIVEIRA, UNIVERSIDADE ESTADUAL PAULISTA; VITOR TRINCA, UNIVERSIDADE ESTADUAL PAULISTA; LOENI LUDKE FALCAO, CENARGEN; LUCILIA HELENA MARCELLINO, CENARGEN; ANTONIO FIGUEIRA, UNIVERSIDADE DE SÃO PAULO; DOUGLAS S. DOMINGUES, UNIVERSIDADE DE SÃO PAULO; ALESSANDRO M. VARANI, UNIVERSIDADE ESTADUAL PAULISTA.
Aparece nas coleções:Artigo em periódico indexado (CPATU)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Integrative-chromosome-scale-genome.pdf4,32 MBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace