Título:	Prevention of renal and genital leptospirosis in experimentally infected ewes by vaccination.
Autoria:	LORIA, J. EZEPHA, C. ROUSSOULIÉRES, I. SOUZA, G. N. de SARDI, F. ALONZO, P. LILENBAUM, W.
Afiliação:	JULIANA LORIA, UNIVERSIDADE FEDERAL FLUMINENSE; CAMILA EZEPHA, UNIVERSIDADE FEDERAL FLUMINENSE; ISABEL ROUSSOULIÉRES, UNIVERSIDADE FEDERAL FLUMINENSE; GUILHERME NUNES DE SOUZA, CNPGL; FLORENCIA SARDI, VIRBAC URUGUAY; PABLO ALONZO, VIRBAC URUGUAI; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE.
Ano de publicação:	2026
Referência:	Next Research, v. 5, 101348, 2026.
Conteúdo:	Infection by Leptospira sp. caused by strains of serogroup Sejroe impairs ruminants’ reproductive efficiency. Control of leptospirosis is complex, and although systemic vaccination minimizes the clinical effects of the disease, the protection against genitourinary colonization is still controversial. Therefore, the present study aims to assess the efficacy of vaccines against L. borgpetersenii serovar Hardjo in preventing renal and genital leptospirosis in experimentally infected ewes. Twenty-four adult Santa Inˆes ewes were divided into four groups (N = 6 each), where Groups 1, 2, and 3 were vaccinated with two doses before challenge (on D-43 and D-15) and Group 4 remained unvaccinated. Groups 1 and 2 received experimental vaccines formulated with the same antigen (L. borgpetersenii sv Hardjo), but with different adjuvants (aluminum hydroxide and mineral emulsion adjuvant, respectively). In contrast, Group 3 received a commercial multivalent vaccine. On D0, all animals were challenged via the genital route with 108 leptospires (L. borgpetersenii serogroup Sejroe, serovar Hardjo). Microscopic Agglutination Test was performed on day D-43, D-15, D0, D7, D14, D21, D30, and D60. Quantitative analysis of urinary and reproductive tract colonization was performed using qPCR of urine and cervicovaginal mucus collected on the same days. The results demonstrate that, although vaccination did not completely prevent kidney colonization, Vac2 (mineral oil) and Vac3 (multivalent) reduced the bacterial load and the number of infected animals throughout the experiment. Regarding genital colonization, Vac2 stimulated an immune response that cleared the infection of all ewes at D30 onwards, presenting an interesting potential for protection of the genital tract.
Thesagro:	Colonização Imunização Leptospirose Ovelha Vacinação
Digital Object Identifier:	https://doi.org/10.1016/j.nexres.2026.101348
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGL)