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dc.contributor.authorRIBEIRO, L. M.
dc.contributor.authorBONATTO, C. C.
dc.contributor.authorSILVA, L. P. da
dc.date.accessioned2026-03-12T17:55:00Z-
dc.date.available2026-03-12T17:55:00Z-
dc.date.created2026-03-12
dc.date.issued2026
dc.identifier.citationPharmaceuticals, v. 19, n. 3, 366, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1185348-
dc.descriptionLiposomes are promising carriers for enhancing antibiotic delivery, but their stability under various stress conditions is crucial for clinical applicability. Methods: This study aimed to evaluate the physicochemical and antimicrobial stability of streptomycin-loaded liposomes subjected to mechanical (bath ultrasonication, probe ultrasonication, ultra-turrax homogenization), thermal (freezing, heating), and chemical (H2O2, Triton X-100, sodium dodecyl sulfate—SDS) stressors. Results: Isolated mechanical stresses (probe ultrasonication, ultrasonic bath, and ultra-turrax) did not significantly affect hydrodynamic diameter (DH), polydispersity index (PdI), or Zeta potential (ZP) (p > 0.05). In contrast, combined ultrasound–freezing stress induced marked destabilization, with DH increasing from ~110 nm to 362 nm (Lc0uf) and from 109 nm to 334 nm (Lc1uf), accompanied by PdI increases from ~0.13 to 0.37–0.41 and a ZP shift in Lc1uf from −43.1 mV to −60.1 mV. Thermal exposure at 75 °C and freezing at −30 °C caused no significant changes in DH or PdI, whereas freezing at −80 °C led to severe destabilization, with over fourfold increases in DH and fivefold increases in PdI; the cholesterol-free formulation (Lc0t−80) reached ~664 nm and a PdI of 0.609. Chemical oxidation with 1% H2O2 did not affect DH, PdI, or ZP, while surfactants (1% Triton X-100 and 1% SDS) significantly altered PdI and ZP in a cholesterol-dependent manner. MIC assays showed that formulations stressed by freezing at −80 °C or by combined ultrasound–freezing retained activity against Escherichia coli (MIC = 50 µg/mL), whereas SDS abolished activity. For Staphylococcus aureus, all stressed formulations remained active, and SDS reduced the MIC from 12.5 to 5.625 µg/mL. Conclusions: These findings demonstrate the robustness and stress-specific stabilization of these liposomal formulations, confirming that the antibiotic’s activity is preserved, which highlights their potential for therapeutic use.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectLiposomes
dc.titleEngineering stability: cholesterol-modulated liposome response to physical and chemical stressors for enhanced antimicrobial activity.
dc.typeArtigo de periódico
dc.subject.thesagroStress
dc.subject.nalthesaurusCholesterol
dc.subject.nalthesaurusStreptomycin
dc.subject.nalthesaurusMicroorganisms
dc.description.notesNa publicação: Luciano Paulino Silva.
riaa.ainfo.id1185348
riaa.ainfo.lastupdate2026-03-12
dc.identifier.doihttps://doi.org/10.3390/ph19030366
dc.contributor.institutionLUÍSA MORATO RIBEIRO, UNIVERSITY OF BRASÍLIA; CÍNTHIA CAETANO BONATTO; LUCIANO PAULINO DA SILVA, CENARGEN.
Aparece en las colecciones:Artigo em periódico indexado (CENARGEN)

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