Título:	Biochemical characterization of a kunitz-type protease inhibitor from mimosa regnellii and its effects on melanoma cell viability and angiogenesis.
Autoria:	RABELO, L. M. A CARDOSO, P. H. de O. NOBRE, L. T. D. B. SANTOS, P. I. M. dos LUCENA, S. V. SERQUIZ, R. P. BEMQUERER, M. P. ROCHA, H. A. de O. NADER, H. B. SANTOS, E. A. dos OLIVEIRA, A. S. de FRIHLING, B. E. F. MIGLIOLO, L.
Afiliação:	LUCIANA MARIA ARAÚJO RABELO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; PEDRO HENRIQUE DE OLIVEIRA CARDOSO, UNIVERSIDADE CATÓLICA DOM BOSCO; LEONARDO THIAGO DUARTE BARRETO NOBRE, UNIVERSIDADE FEDERAL DE SÃO PAULO; PAULA IVANI MEDEIROS DOS SANTOS, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; SHEYLA VARELA LUCENA, INSTITUTO FEDERAL DE CIÊNCIAS E TECNOLOGIA DO RIO GRANDE DO NORTE; RAPHAEL PASCHOAL SERQUIZ, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; MARCELO PORTO BEMQUERER, CNPGL; HUGO ALEXANDRE DE OLIVEIRA ROCHA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; HELENA BONCIANI NADER, UNIVERSIDADE FEDERAL DE SÃO PAULO; ELIZEU ANTUNES DOS SANTOS, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; ADELIANA SILVA DE OLIVEIRA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; BRENO EMANUEL FARIAS FRIHLING, UNIVERSIDADE CATÓLICA DOM BOSCO; LUDOVICO MIGLIOLO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE.
Ano de publicação:	2026
Referência:	Chemistry & Biodiversity, v. 23, e03760, 2026.
Conteúdo:	Cancer is a term used to represent more than 100 diseases, including malignant tumors of different localizations. Among these types, cutaneous melanoma is one of the most common types of skin cancer worldwide. Newer, non-invasive, and more effective forms of treatment are urgently needed. Recently, the cancer membrane has emerged as a novel target for new anticancer drugs. Serine protease inhibitors are researched across all kingdoms; however, the Plantae kingdom is a more sustainable producer of bioactive molecules, including serine protease inhibitors. In this study, a Kunitz trypsin inhibitor (JTI) purified by RP-HPLC from Mimosa regnellii seeds was sequenced, its inhibitory activity quantified, and its inhibition constant determined. It was then tested for its ability to induce cell death via the apoptotic pathway in B16-F10 mouse melanoma cells. To evaluate its pro-apoptotic capacity, the inhibitor’s effects on reactive oxygen speciesrelease, calcium release, cell morphological changes, and the inhibition of angiogenic and migratory activities of rabbit endothelial cells (RaEC) were also assessed in vitro. Tests for toxicity against normal cells were performed, confirming that JTI acts on melanoma cells with an IC50 of 0.65 µM. These results suggest that JTI has potential for use in melanoma treatment.
Thesagro:	Protease Enzima Tripsina
NAL Thesaurus:	Melanoma
Palavras-chave:	Angiogênese Migração celular Apoptose
Digital Object Identifier:	https://doi.org/10.1002/cbdv.202503760
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGL)