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dc.contributor.authorSILVA, J. de J.
dc.contributor.authorSILVA, J. C. da
dc.contributor.authorGOMES, R. A.
dc.contributor.authorOLIVEIRA, V. R. de
dc.contributor.authorDANTAS, B. F.
dc.date.accessioned2026-05-19T16:49:56Z-
dc.date.available2026-05-19T16:49:56Z-
dc.date.created2026-05-19
dc.date.issued2026
dc.identifier.citationJournal of Seed Science, v. 48, e202648006, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1186952-
dc.descriptionRapid tests are essential for assessing the physiological quality of seeds, especially in native Caatinga species that remain little studied. Cnidoscolus quercifolius (Pohl) has ecological and socioeconomic importance but lacks protocols for determining viability. This study aimed to standardize the tetrazolium test (TZT) for evaluating C. quercifolius seeds. Three seed lots were used, from Massaroca (BA), Sento Sé (BA), and Petrolina (PE). The seeds were preconditioned for 16 h at 30 °C on moistened paper and then had the seed coat removed. The experiment followed a completely randomized design in a 7 × 4 factorial arrangement, with seven concentrations of 2,3,5 triphenyl tetrazolium chloride (0.025 to 0.75%) and four immersion times (1 to 4 h), with four replications of 25 seeds. Viability was assessed by the intensity and uniformity of carmine-red staining in the embryo and cotyledons. Low tetrazolium chloride concentrations (≤ 0.025%) underestimated viability, whereas high ones (≥ 0.5%) caused overstaining. Thus, thee range of 0.05 to 0.075% for 3-4 h showed greater diagnostic efficiency, reflecting germination percentages. TZT distinguished viable seeds, with uniform staining and turgid tissues, from non-viable ones. It is concluded that TZT is rapid, reliable, and applicable to programs for conservation, seedling production, and ecological restoration in the Caatinga.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectTeste bioquímico
dc.subjectSementes nativas
dc.subjectQualidade fisiológica
dc.titleDevelopment of a specific protocol for the tetrazolium test and evaluation of the physiological potential of Cnidoscolus quercifolius Pohl (Euphorbiaceae) seeds.
dc.typeArtigo de periódico
dc.subject.thesagroSemente
dc.subject.thesagroFaveleira
dc.subject.thesagroCaatinga
dc.subject.thesagroEspécie Nativa
dc.subject.nalthesaurusCnidoscolus quercifolius
riaa.ainfo.id1186952
riaa.ainfo.lastupdate2026-05-19
dc.identifier.doihttp://dx.doi.org/10.1590/2317-1545v48302327
dc.contributor.institutionJAILTON DE JESUS SILVA; JAMILLE CARDEAL DA SILVA, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; RAQUEL ARAUJO GOMES, UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA; VISELDO RIBEIRO DE OLIVEIRA, CPATSA; BARBARA FRANCA DANTAS, CPATSA.
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