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dc.contributor.authorVELOSO, D. S.
dc.contributor.authorARAUJO, F. R.
dc.contributor.authorSANTOS, L. R. dos
dc.contributor.authorSUNIGA, P. A. P.
dc.contributor.authorMORIYA, J. C. K.
dc.contributor.authorVERBISCK, N. V.
dc.contributor.authorGASQUÉ, M.
dc.contributor.authorLAROUCAU, K.
dc.contributor.authorLEMOS, E. R. S.
dc.contributor.authorHORTA, M. A.
dc.date.accessioned2026-05-19T18:48:39Z-
dc.date.available2026-05-19T18:48:39Z-
dc.date.created2026-05-19
dc.date.issued2026
dc.identifier.citationInfection, Genetics and Evolution, v. 139, e105915, 2026.
dc.identifier.issn1567-7257
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1186955-
dc.descriptionABSTRACT - Burkholderia pseudomallei is a Gram-negative facultative intracellular bacterium that causes melioidosis, a neglected tropical disease that spreads beyond traditional endemic regions. This study examined B. pseudomallei isolates obtained from five melioidosis cases in Piauí, Brazil, using phenotypic, molecular, and genomic methods. Isolates were identified as B. pseudomallei using VITEK 2 system and MALDI-TOF mass spectrometry. Conventional PCR targeting the orf11, burk17, and burk475 gene regions confirmed species-level identification, with sequences showing 98.78–100% identity to GenBank B. pseudomallei references. Whole-genome sequencing and multi-locus sequence typing (MLST) revealed two novel sequence types; ST2226 (four isolates), clustering closely with strains from Cear´ a (ST1455 and ST1458), ST2234 (one isolate), related to previously reported ST95 and ST1355 strains, previously detected in Brazil. Phylogenetic analysis based on single nucleotide polymorphism (SNPs) and concatenated MLST data showed clear genetic distinction between the two lineages, suggesting regional spread or shared ancestry. These findings confirm the presence of at least two distinct genetic B. pseudomallei lineages in Piauí, providing insights into bacterial diversity and regional epidemiology in Brazil and reinforcing the importance of molecular surveillance and improved diagnostic capacity for melioidosis.
dc.language.isoeng
dc.rightsopenAccess
dc.titleEmergence of distinct Burkholderia pseudomallei lineages in Brazil: Phenotypic, genotypic, and phylogenetic agreement.
dc.typeArtigo de periódico
dc.subject.nalthesaurusBurkholderia pseudomallei
dc.subject.nalthesaurusMelioidosis
dc.subject.nalthesaurusMass spectrometry
dc.format.extent211 p.
riaa.ainfo.id1186955
riaa.ainfo.lastupdate2026-05-19
dc.identifier.doi10.1016/j.meegid.2026.105915
dc.contributor.institutionDILBERT SILVA VELOSO, UNIVERSIDADE FEDERAL DO PIAUI; FLABIO RIBEIRO DE ARAUJO, CNPGC; LENITA RAMIRES DOS SANTOS, CNPGC; PAULA ADAS PEREIRA SUNIGA; JÉSSICA CRISTINE KURAMOTO MORIYA; NEWTON VALERIO VERBISCK, CNPGC; MÉGANE GASQUÉ, UNIVERSIDADE DE PARIS; KARINE LAROUCAU, UNIVERSIDADE DE PARIS; ELBA REGINA SAMPAIO LEMOS, FUNDAÇÃO OSWALDO CRUZ; MARCO AURELIO HORTA, FUNDAÇÃO OSWALDO CRUZ.
Aparece nas coleções:Artigo em periódico indexado (CNPGC)

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