Título:	From deforestation to regeneration: How do land-use changes shape soil microbes and methane-cycling genes in the Eastern Amazon?
Autoria:	MANDRO, J. A. GONTIJO, J. B. NAKAMURA, F. M. BORGES, C. D. OLIVEIRA JUNIOR, R. C. de BERENGUER, E. BOHANNAN, B. J. NÜSSLEIN, K. RODRIGUES, J. L. M. TSAI, S. M. VENTURINI, A. M.
Afiliação:	JÉSSICA A. MANDRO, UNIVERSIDADE DE SÃO PAULO; JÚLIA B. GONTIJO, UNIVERSIDADE DE SÃO PAULO; FERNANDA M. NAKAMURA, UNIVERSIDADE DE SÃO PAULO; CLOVIS D. BORGES, UNIVERSIDADE DE SÃO PAULO; RAIMUNDO COSME DE OLIVEIRA JUNIOR, CPATU; ERIKA BERENGUER, UNIVERSITY OF OXFORD; BRENDAN J.M. BOHANNAN, UNIVERSITY OF OREGON; KLAUS NÜSSLEIN, UNIVERSITY OF MASSACHUSETTS; JORGE L. MAZZA RODRIGUES, UNIVERSITY OF CALIFORNIA; SIU M. TSAI, UNIVERSIDADE DE SÃO PAULO; ANDRESSA M. VENTURINI, UNIVERSIDADE DE SÃO PAULO.
Ano de publicação:	2026
Referência:	Total Environment Microbiology, v. 2, n. 1, 100069, 2026.
Conteúdo:	Land-use change in the Amazon Rainforest impacts soil properties and belowground microbial communities, with far-reaching implications for soil ecosystem services, including methane cycling processes. However, it is unclear whether the known methane sink-to-source shift observed after forest-to-pasture conversion occurs consistently throughout the year, or whether forest regeneration can help restore this crucial ecosystem process. Here, we assessed the impacts of forest-to-pasture conversion and forest regeneration in the Amazon Rainforest on its soil properties and microbial communities, focusing on methane-related microbiota, using 16S rRNA sequencing and quantitative real-time PCR. Conversion resulted in significant changes in soil chemistry and microbial communities, while seasonality and its interaction with land use intensified these differences. Land-use change also increased the abundance of methanotrophs and methanogens, but the ratio between both groups was altered, consistent with pastures as potential sources of methane and forests as sinks. Seasonality further increased the impact of the conversion on methane-cycling microorganisms. Our findings also highlight the potential of passive forest regeneration to restore certain soil chemical and microbiological patterns similar to those of primary forests, including methane-related genes. Although these results provide strategies to support methane mitigation, they indicate that the functional and taxonomic potential of microbial communities may not be equally recovered. This highlights the importance of maintaining primary forests to preserve critical ecosystem services.
Thesagro:	Floresta Secundaria
Palavras-chave:	Recuperação Conversão de floresta em pastagem
ISSN:	3050-6417.
Digital Object Identifier:	https://doi.org/10.1016/j.temicr.2026.100069
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATU)