Título:	Dynamics of bacterial communities and resistomes across swine waste stabilization ponds and fertilized soils.
Autoria:	ALEGRIA, O. V. C. TORRES, M. C. BREYER, G. M. REBELATTO, R. WUADEN, C. R. PASTORE, J. LAZZAROTTI, M. RAMOS, R. T. J. DORN, M. KICH, J. D. SIQUEIRA, F. M.
Afiliação:	OSCAR VICTOR CARDENAS ALEGRIA, UNIVERSIDADE FEDERAL DO PARÁ; MARIANA COSTA TORRES, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; GABRIELA MERKER BREYER, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; RAQUEL REBELATTO, CNPSA; CAMILA ROSANA WUADEN; JANAINA PASTORE; MATEUS LAZZAROTTI, CNPSA; ROMMEL THIAGO JUCA RAMOS, UNIVERSIDADE FEDERAL DO PARÁ; MARCIO DORN, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; JALUSA DEON KICH, CNPSA; FRANCIELE MABONI SIQUEIRA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL.
Ano de publicação:	2026
Referência:	Current Microbiology, v. 83, n. 8, p. 0343-8651, 2026.
Conteúdo:	The environmental dissemination of antimicrobial resistance (AMR) through livestock waste represents a growing concern for human, environmental, and animal health. This study investigated how swine waste stabilization ponds (WSPs), and subsequent manure application to agricultural soils, influence bacterial community structure, antimicrobial resistance genes (ARGs), and mobile genetic elements (MGEs). Using shotgun metagenomics, we analyzed 80 samples from 20 swine farms, including waste collected before and after WSP treatment and soils with and without a history of manure application. Distinct microbial profiles were observed between waste and soil environments. Waste samples were dominated by Bacillota, Bacteroidota, and Pseudomonadota, whereas soils were enriched in Actinomycetota, particularly Streptomyces. WSP significantly reduced microbial diversity and caused shifts toward stress-tolerant taxa, indicating selective pressures during the process. Manure-fertilized soils exhibited altered community composition and enrichment of clinically relevant ARGs, including the fluoroquinolone resistance gene adeF. Waste management practices influenced resistome composition, with treated waste showing increased relative abundance of macrolide resistance genes (ermB and mefA). In soils, ARG profiles were associated with distinct MGE patterns, suggesting environment-specific mechanisms of gene mobility. Phage-associated elements were more prevalent in waste samples, whereas transposons were more prominent in soils, where ARG-MGE co-occurrence patterns indicated potential for horizontal gene transfer. Overall, our findings demonstrate that WSP management and soil application of swine manure shape both microbial communities and resistome configurations. These results underscore the importance of integrating waste treatment strategies into AMR surveillance frameworks and support a One Health approach to mitigate its dissemination in agroecosystems.
Thesagro:	Suinocultura Tratamento do Solo Águas Residuais Manejo Dejeto Biofertilizante Adubação
Palavras-chave:	Resistoma
Digital Object Identifier:	https://doi.org/10.1007/s00284-026-05026-6
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPSA)