Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1188057
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dc.contributor.authorSILVEIRA, K. R. D.
dc.contributor.authorHAACH, V.
dc.contributor.authorBASTOS, A. P. A.
dc.date.accessioned2026-07-07T13:49:19Z-
dc.date.available2026-07-07T13:49:19Z-
dc.date.created2026-07-07
dc.date.issued2026
dc.identifier.citationFoods, v. 15, n. 12, p. 2218, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1188057-
dc.descriptionAbstract: Cultivated-meat production relies on robust animal cell-line engineering, scalable tissue-engineering strategies, and clearly defined regulatory standards. This review examines the developmental pipeline from primary tissue biopsy to large-scale expansion and regulatory evaluation, focusing on stable and safe immortalized cell platforms. We compare muscle satellite cells, mesenchymal stromal/adipogenic progenitors and induced pluripotent stem cells, highlighting trade-offs among proliferative capacity, lineage commitment, genomic stability, and food-safety considerations. We then analyze immortalization strategies, including spontaneous senescence bypass, telomerase reactivation and CRISPR-based checkpoint modulation, highlighting their impact on genomic stability and food-safety risks. Recent advances in serum-free media, extracellular matrix-mimetic biomaterials and staged co-culture protocols have enabled centimeter-scale tissues with improved texture and marbling; however, cost, reproducibility and scalability remain bottlenecks. Integrating multi-omics surveillance with life-cycle assessment reveals that environmental benefits (land, water and antibiotic reduction) are attainable only when energy inputs and growth-factor sourcing are optimized. Finally, we examine regulatory frameworks that distinguish food-grade immortalized cells from pharmaceutical substrates and genetically modified crops. By integrating cell biology, animal biotechnology, and bioprocess engineering, this review identifies technical priorities for advancing cultivated meat from laboratory development to industrial implementation, positioning genomic monitoring as an essential framework for assessing biological stability, functional predictability, and food-production suitability.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectCarne cultivada
dc.subjectLinhagens celulares animais
dc.subjectCélulas primárias
dc.subjectImortalização
dc.subjectEstabilidade genômica
dc.subjectDiferenciação miogênica
dc.subjectCultivated meat
dc.subjectAnimal cell lines
dc.subjectPrimary cells
dc.subjectImmortalization
dc.subjectGenomic stability
dc.subjectMyogenic differentiation
dc.titleGenomic monitoring and engineering stable and safe immortalized cell platforms for industrial cellular agriculture.
dc.typeArtigo de periódico
dc.subject.thesagroProdução de Carne
riaa.ainfo.id1188057
riaa.ainfo.lastupdate2026-07-07
dc.identifier.doihttps://doi.org/10.3390/foods15122218
dc.contributor.institutionKARINE R. D. SILVEIRA; VANESSA HAACH; ANA PAULA ALMEIDA BASTOS, CNPSA.
Aparece nas coleções:Artigo em periódico indexado (CNPSA)

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