Título:	UPLC-ESI-QToF-MS/MS profiling and anxiolytic-like effects of Simira paraensis (Baill.) Steyerm bark.
Autoria:	MARTINS, L. R. J. PARACAMPO, N. E. N. P. RIBEIRO, P. R. V. OIRAM FILHO, F. ALVES, A. M. B. SANTOS, H. S. dos MENEZES, J. E. S. A. de MARINHO, M. M. ROCHA, M. N. da MARINHO, E. S. CANUTO, K. M.
Afiliação:	LARISSA RODRIGUES JALES MARTINS, UNIVERSIDADE ESTADUAL DO CEARÁ; NADIA ELIGIA NUNES PINTO PARACAMPO, CPATU; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; FRANCISCO OIRAM FILHO; AMANDA MARIA BARROS ALVES, UNIVERSIDADE ESTADUAL DO CEARÁ; HÉLCIO SILVA DOS SANTOS, UNIVERSIDADE ESTADUAL DO CEARÁ; JANE EIRE SILVA ALENCAR DE MENEZES, UNIVERSIDADE ESTADUAL DO CEARÁ; MÁRCIA MACHADO MARINHO, UNIVERSIDADE ESTADUAL DO CEARÁ; MATHEUS NUNES DA ROCHA, UNIVERSIDADE ESTADUAL DO CEARÁ; EMMANUEL SILVA MARINHO, UNIVERSIDADE ESTADUAL DO CEARÁ; KIRLEY MARQUES CANUTO, CNPAT.
Ano de publicação:	2026
Referência:	Journal of Pharmaceutical and Biomedical Analysis, v. 280, 117602, 2026.
Conteúdo:	Simira paraensis (Baill.) Steyerm (Rubiaceae), popularly known as ararib´a-rosa, is a small tree found mainly in the Amazon. Studies on the genus Simira report a chemical composition rich in indole alkaloids of the β-carboline class, which are associated with effects on the central nervous system. The present study aimed to characterize the chemical profile and evaluate the anxiolytic activity of S. paraensis bark through in vivo and in silico assays. The powdered bark was extracted with a hydromethanolic solution, yielding a red-colored extract (SP-EMB), which was analyzed by Ultra High Performance Liquid Chromatography coupled to Electrospray Ionization Mass Spectrometry and Quadrupole-Time-of-Flight Analyzer (UPLC-ESI-QToF-MS/MS). The neuropharmacological activity was evaluated in zebrafish (Danio rerio) using locomotor activity, light/dark, novel tank, GABAergic neuromodulation, and PTZ-induced seizure assays. UPLC-ESI-QToF-MS/MS analysis revealed 15 β-carboline alkaloids, 4 phenolic acid derivatives, and 1 phenolic glycoside. Harmane, the major constituent, was isolated by preparative HPLC and identified by UPLC-ESI-QToF-MS/MS and NMR analyses. SP-EMB exhibited anxiolytic-like effects in both light/dark and novel tank tests, with low acute toxicity (LD₅₀ > 400 mg⋅kg⁻¹) and reduced sedative effects compared to diazepam (DZP, 4 mg⋅kg⁻¹). Molecular docking showed that harmane and chlorogenic acid have high affinity for the GABAA receptor, indicating their influence on the anxiolytic effect. Both compounds showed low toxicity (LD₅₀ > 40 mg⋅kg⁻¹) and dose-dependent anxiolytic-like activity without locomotor impairment. Furthermore, chlorogenic acid exhibited anxiolytic effects mediated by GABAA receptors. These findings may suggest that S. paraensis is a promising alternative source of anxiolytic agents with lower sedative effects.
Thesagro:	Arariba
Palavras-chave:	Simira paraensis Ansiolítico Propriedade quimica
ISSN:	0731-7085.
Digital Object Identifier:	https://doi.org/10.1016/j.jpba.2026.117602
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPATU)