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dc.contributor.authorSOUZA, L. dos R. de
dc.contributor.authorSOUZA, M. F. S.
dc.contributor.authorLIMA, P. A.
dc.contributor.authorCIRILO, T. M.
dc.contributor.authorRIBEIRO, L. N.
dc.contributor.authorCARVALHO, S. A. P.
dc.contributor.authorGUIMARÃES, A. S.
dc.contributor.authorSOUZA, P. G. de
dc.contributor.authorSOARES FILHO, P. M.
dc.contributor.authorCUNHA, J. L. R.
dc.contributor.authorBUENO, L. L.
dc.contributor.authorPAIXÃO, T. A. da
dc.contributor.authorFUJIWARA, R. T.
dc.contributor.authorSANTOS, R. L.
dc.date.accessioned2026-07-22T16:48:54Z-
dc.date.available2026-07-22T16:48:54Z-
dc.date.created2026-07-22
dc.date.issued2026
dc.identifier.citationPLoS One, v. 21, n. 6, e0352788, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1188549-
dc.descriptionSerological methods are valuable diagnostic tools for bovine brucellosis, a zoonotic infectious disease with a worldwide distribution. Currently employed diagnostic methods utilize crude bacterial extract or regions of LPS, a molecule that can exhibit cross-reactions with other infectious agents. The goal of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISAi) using synthetic peptides or a multi-epitope protein based on in silico-predicted B cell epitopes. Peptides were synthesized on a cellulose membrane using spot synthesis, and immunoblots were performed to evaluate reactivity by densitometry against bovine sera of interest. Peptides reacting with serum from positive cattle and non-reactive to the serum from negative controls were selected, synthesized in a soluble form, and used as antigens for the development of the ELISAi. Two peptides (P1 and P2) were selected and, after standardization of the ELISAi, positive (25) and negative (175) samples were tested, resulting in a sensitivity of 84% (21/25) and specificity of 83.43% (146/175). The sequence of the two peptides in replicates with spacers were inserted into a multi-epitope protein, which was also used as antigen in an ELISAi resulting in a sensitivity of 72% (18/25) and a specificity of 61.71% (108/175). This study provided a preliminary analytical assessment of in silico-predicted epitopes for developing novel serologic diagnostic tests.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectImunoabsorção enzimática
dc.titleIn silico-predicted B-cell epitopes for bovine brucellosis serodiagnosis: Preliminary analytical evaluation of synthetic peptide- and multi-epitope protein-based indirect ELISAs.
dc.typeArtigo de periódico
dc.subject.thesagroBovino
dc.subject.thesagroDoença Animal
dc.subject.thesagroBrucelose
riaa.ainfo.id1188549
riaa.ainfo.lastupdate2026-07-22
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0352788
dc.contributor.institutionLUCAS DOS REIS DE SOUZA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; MONIQUE FERREIRA SILVA SOUZA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PÂMELA APARECIDA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; TATYANE MARTINS CIRILO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; LETÍCIA NEVES RIBEIRO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; SAMUEL ALEXANDRE PIMENTA CARVALHO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ALESSANDRO DE SA GUIMARAES, CNPGL; PATRÍCIA GOMES DE SOUZA, MINISTÉRIO DA AGRICULTURA E PECUÁRIA; PAULO MARTINS SOARES FILHO, MINISTÉRIO DA AGRICULTURA E PECUÁRIA; JOÃO LUÍS REIS CUNHA, UNIVERSITY OF YORK; LILIAN LACERDA BUENO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; TATIANE ALVES DA PAIXÃO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RICARDO TOSHIO FUJIWARA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RENATO LIMA SANTOS, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
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