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dc.contributor.authorSANTOS, A. L. M. dos
dc.contributor.authorLEITAO, R. C.
dc.contributor.authorCAMILLO, L. de M. B.
dc.contributor.authorSANTOS, G. B. dos
dc.contributor.authorSANTOS, A. B. dos
dc.date.accessioned2026-07-29T13:54:51Z-
dc.date.available2026-07-29T13:54:51Z-
dc.date.created2026-07-29
dc.date.issued2026
dc.identifier.citationBioresource Technology, v. 453, 134624, Aug. 2026.
dc.identifier.issn1873-2976
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1188697-
dc.descriptionBrewery spent grain (BSG) is a promising substrate for anaerobic digestion (AD). However, its lignocellulosic structure limits biodegradability. Steam explosion (SE) pretreatment enhances solubilization but may also generate inhibitory compounds, including free ammonia nitrogen (FAN), volatile fatty acids (VFAs), and p-cresol. This study evaluated the AD of BSG mixtures containing 0–100% SE hydrolysate in 85-day batch assays at 37 °C, assessing methane (CH4) production, inhibition dynamics, microbial community shifts, and predicted functional responses inferred from taxonomic profiles. The mixture containing 50% hydrolysate (EXP-0.5) exhibited the least compromised performance, with a CH4 yield of 101.0 ± 4.2 mL CH4/gVSadded and a reduced lag phase (λ = 1.7 d). Increasing hydrolysate proportions intensified inhibitory conditions, with FAN concentrations exceeding 500 mg/L, acetic acid reaching 2448 mg/L, and p-cresol concentrations surpassing 280 mg/L. These conditions may have constrained key oxidation steps and, consequently, contributed to reduced CH4 production. Despite these stresses, Methanothrix remained the dominant methanogen, suggesting a persistent predominance of acetoclastic methanogenesis, albeit with reduced functional efficiency. Functional predictions for EXP-0.5 suggested a higher representation of pathways related to the citrate cycle, carbohydrate, and amino acid metabolism, implying a potential for sustained energy flux and adaptive responses to ammonia stress. Overall, the results infer that moderate hydrolysate incorporation can attenuate, but not eliminate, inhibition of SE-treated BSG during AD. Therefore, integrated strategies in lignocellulosic biorefinery schemes using SE hydrolysates are required to balance the benefits of enhanced solubilization with microbial metabolic resilience and long-term process stability in AD systems.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectLignocellulosic biomass
dc.subjectSteam explosion pretreatment
dc.subjectAnaerobic digestion inhibition
dc.subjectFunctional metabolic pathways
dc.subjectMiDAS_g_606
dc.subjectBiomassa lignocelulósica
dc.subjectPré-tratamento por explosão a vapor
dc.subjectInibição da digestão anaeróbia
dc.subjectVias metabólicas funcionais
dc.titleFunctional insights into inhibition by steam-exploded brewery spent grain hydrolysate in anaerobic digestion: roles of ammonia, volatile fatty acids, and phenolics.
dc.typeArtigo de periódico
riaa.ainfo.id1188697
riaa.ainfo.lastupdate2026-07-29
dc.identifier.doihttps://doi.org/10.1016/j.biortech.2026.134624
dc.contributor.institutionAMANDA LIMA MORAES DOS SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; RENATO CARRHA LEITAO, CNPAT; LÍVIA DE MORAES BOMEDIANO CAMILLO, UNIVERSIDADE FEDERAL DO ABC; GABRIELI BOVI DOS SANTOS, UNIVERSIDADE FEDERAL DO ABC; ANDRÉ BEZERRA DOS SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ.
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