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dc.contributor.authorLIZ, R. D. de
dc.contributor.authorNOGUEIRA, G. F.
dc.contributor.authorFERMINO JÚNIOR, P. C. P.
dc.contributor.authorPEREIRA, J. E. S.
dc.contributor.authorCAMPOS, J. M. S. de
dc.contributor.authorSILVA-CARDOSO, I. M. de A.
dc.contributor.authorDAL VESCO, L. L.
dc.contributor.authorPESCADOR, R.
dc.date.accessioned2026-07-30T17:49:02Z-
dc.date.available2026-07-30T17:49:02Z-
dc.date.created2026-07-30
dc.date.issued2026
dc.identifier.citationBiologia Plantarum, v. 70, p. 99-108, 2026.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1188770-
dc.descriptionBackground: Protocorm-like bodies (PLBs) represent a major morphogenic pathway in orchids and are characterized by cellular reprogramming, tissue differentiation, and developmental reorganization. Although PLB formation is widely explored for orchid micropropagation, endopolyploidy dynamics during PLB morphogenesis remain poorly understood. Aims: This study aimed to characterize endopolyploidy patterns and evaluate nuclear DNA content maintenance during PLB morphogenesis and plant regeneration in Cattleya tigrina. Methods: Basal leaf explants were cultured under PLB-inducing conditions in vitro. Morphological and histological analyses were performed throughout PLB development, while nuclear DNA content was estimated by flow cytometry in leaf regions, PLBs at successive developmental stages, and regenerated plants. Results: The leaf base was the most responsive region for PLB induction, showing intense cell proliferation and meristematic activity. Flow cytometric profiles consistently revealed 2C, 4C, and 8C nuclear DNA peaks, indicating endopolyploidy in somatic tissues. PLBs exhibited transient shifts in ploidy distribution during active proliferation phases, particularly at intermediate developmental stages, when 4C nuclei became predominant. Regenerated plants maintained nuclear DNA contents comparable to the original explants. Conclusions: Endopolyploidy is an intrinsic and developmentally regulated feature of PLB morphogenesis in Cattleya tigrina. PLB-mediated regeneration maintained overall nuclear DNA content under the evaluated in vitro conditions.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectPloidy stability
dc.subjectProtocorm-like bodies
dc.titleEndopolyploidy and ploidy stability during protocorm-like body morphogenesis and regeneration in Cattleya tigrina A. Richard.
dc.typeArtigo de periódico
dc.subject.thesagroOrchidaceae
dc.subject.nalthesaurusEndopolyploidy
dc.subject.nalthesaurusFlow cytometry
dc.subject.nalthesaurusMorphogenesis
dc.description.notesNa publicação: Jonny Everson Scherwinski-Pereira.
riaa.ainfo.id1188770
riaa.ainfo.lastupdate2026-07-30
dc.identifier.doi10.32615/bp.2026.011
dc.contributor.institutionRAFAELA DUARTE DE LIZ, FEDERAL UNIVERSITY OF SANTA CATARINA; GABRIELA FERREIRA NOGUEIRA; PAULO CÉSAR POETA FERMINO JÚNIOR, FEDERAL UNIVERSITY OF SANTA CATARINA; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN; JOSÉ MARCELLO SALABERT DE CAMPOS, FEDERAL UNIVERSITY OF JUIZ DE FORA; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; LÍRIO LUIZ DAL VESCO, FEDERAL UNIVERSITY OF SANTA CATARINA; ROSETE PESCADOR, FEDERAL UNIVERSITY OF SANTA CATARINA.
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