Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/12425
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dc.contributor.authorCAPALBO, D. M. F.pt_BR
dc.contributor.authorARANTES, O. N.pt_BR
dc.contributor.authorMEDUGNO, C. C.pt_BR
dc.contributor.authorSILOTO, R. C.pt_BR
dc.contributor.authorWATANABE, M. A.pt_BR
dc.contributor.authorGONZALES, G. M.pt_BR
dc.date.accessioned2017-02-16T22:45:08Z-
dc.date.available2017-02-16T22:45:08Z-
dc.date.created1996-03-07pt_BR
dc.date.issued1994pt_BR
dc.identifier.citationIn: THE PACIFIC RIM CONFERENCE ON BIOTECHNOLOGY OF BACILLUS THURINGIENSIS AND ITS IMPACT TO THE ENVIRONMENT, 1994, Taipei. Program and Abstracts. Taipei: Academia Sinica, 1994.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/12425pt_BR
dc.descriptionA loopful of Bti mantained on Luria-Bertani agar medium was transferred to Bacto-Peptona agar medium. After 3 days at 30oC, the petri-disheswere washed with sterilized water (100ml/petri-dish). The suspension obtained represented the inoculum for the semi-solid rice-based fermentation medium. The growth units were incubated until complete sporulation of Bti. The resulting biomass was dried at 60oC in an oven for 24h, sieved through 60 tyler. The retained powder did not show any larvicidal activity. In parallel, colloidal bentonite was treated with organic macromolecules dissolved in toluene, after sedimentation, the botton phase was dried at 120oC for 4h. This treatment led to a spreading and floating clay which resulted in a good carrier for Bti. Then, the the two powders were mixed in the proportion of 1(Bti):150(clay). The bioassays were performed under laboratory conditions utilizing 2nd instar Culex sp. larvae, with 3 repetitions (20 larvae each) being observed during 96 hours. The data were treated with Abbott method. A complete methodology was developed to get floating units of Bti with 143 mg/m2 of biological activity.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBacillus thuringiensis varpt_BR
dc.subjectisraelensispt_BR
dc.subjectRice mediumpt_BR
dc.subjectFermentation mediumpt_BR
dc.subjectFormulationpt_BR
dc.subjectBiopesticidept_BR
dc.subjectBiopesticidapt_BR
dc.titleFloating formulation for Bacillus thuringiensis var. israelensis obtained from semi-solid rice medium.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2017-02-16T22:45:08Zpt_BR
dc.subject.thesagroArrozpt_BR
dc.subject.thesagroControle Biológicopt_BR
dc.subject.thesagroFermentaçãopt_BR
dc.subject.thesagroFormulaçãopt_BR
dc.subject.thesagroMeio de Culturapt_BR
dc.subject.nalthesaurusbiological controlpt_BR
dc.format.extent2p.72pt_BR
riaa.ainfo.id12425pt_BR
riaa.ainfo.lastupdate2017-02-16pt_BR
dc.contributor.institutionDEISE MARIA FONTANA CAPALBO, CNPMA; UEL; CLAUDIA CONTI MEDUGNO, CNPMA; CNPMA; MARIA AICO WATANABE, CNPMA; PETROBRAS.pt_BR
Appears in Collections:Resumo em anais de congresso (CNPMA)

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