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dc.contributor.authorSCRAMIN, S.eng
dc.contributor.authorROQUE, M. R. A.eng
dc.contributor.authorDENTZIEN, A. F. M.eng
dc.contributor.authorROSSO, C. R. S. deeng
dc.contributor.authorMELO, I. S. deeng
dc.date.accessioned2019-05-08T00:35:29Z-
dc.date.available2019-05-08T00:35:29Z-
dc.date.created1998-05-26
dc.date.issued1998
dc.identifier.citationIn: LATIN AMERICAN BIODEGRADATION & BIODETERIORATION SYMPOSIUM, 3., 1998, Florianopolis. Resumos. Florianopolis: UFSC, 1998.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/12899-
dc.descriptionTwo Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectExtracellular enzymeseng
dc.subjectAcinetobacter sppeng
dc.titlePresence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers.eng
dc.typeResumo em anais e proceedingseng
dc.date.updated2019-05-08T00:35:29Z
dc.format.extent2p.16eng
riaa.ainfo.id12899eng
riaa.ainfo.lastupdate2019-05-07
dc.contributor.institutionSHIRLEI SCRAMIN, EMBRAPA-CNPMA; ANAMARIA FERREIRA MAYER DENTZIEN, CNPMA; CESAR ROBERTO SILVA DE ROSSO, CNPMA; ITAMAR SOARES DE MELO, CNPMA.eng
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