Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/137733
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dc.contributor.authorMELO, N. F. de
dc.contributor.otherNATONIEL FRANKLIN DE MELO, CPATSA.
dc.date.accessioned2018-05-15T00:33:28Z-
dc.date.available2018-05-15T00:33:28Z-
dc.date.created2003-03-14
dc.date.issued2002
dc.identifier.other25936
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/137733-
dc.descriptionThe regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number.
dc.description.uribitstream/item/176744/1/Separata-9620.pdfpt_BR
dc.languageen
dc.language.isoengeng
dc.publisherCytologia, Tpkyo, v. 67, n. 4, p. 337-341, 2002.
dc.relation.ispartofEmbrapa Semiárido - Artigo em periódico indexado (ALICE)
dc.titleSomatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves.
dc.typeArtigo em periódico indexado (ALICE)
dc.date.updated2018-05-15T00:33:28Zpt_BR
dc.subject.thesagroAnálise
dc.subject.thesagroCalo
dc.subject.thesagroCitogenética Vegetal
dc.subject.thesagroEmbriogénese
dc.subject.thesagroMandioca
dc.subject.thesagroManihot Esculenta
dc.subject.thesagroMicropropagação
dc.subject.thesagroVariedade
dc.ainfo.id137733
dc.ainfo.lastupdate2018-05-14
Appears in Collections:Artigo em periódico indexado (CPATSA)

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