Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/137733
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorMELO, N. F. de
dc.date.accessioned2018-05-15T00:33:28Z-
dc.date.available2018-05-15T00:33:28Z-
dc.date.created2003-03-14
dc.date.issued2002
dc.identifier.citationCytologia, Tpkyo, v. 67, n. 4, p. 337-341, 2002.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/137733-
dc.descriptionThe regeneration and cytogenetic analysis of 7 cassava (Manihot esculenta Crantz) cultivars obtained by organogenesis and somatic embryogenesis were investigated. In the first phase, callus formation was induced in young leaves using MS medium supplemented with 1.0, 5.0, 10.0, 15.0 and 20.0 mg/l of 2,4-D or without a growth regulator (control). In the second phase (development), the calli obtained in the above treatments were transferred to MS medium supplemented with factorial combinations of GA3 and BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/l. Results of the first phase showed that 2,4-D induced the formation of both friable and compact calli. The compact calli displayed root and shoot formation, whilst somatic embryos were obtained only from friable calli in the medium with 1.0 mg/l GA3. Plants obtained from somatic embryos were regenerated in MS medium supplemented with 0.04 mg/l NAA and 0.05 mg/l GA3. Cytogenetic analysis in 167 regenerated plants from somatic embryogenesis and 176 from organogenesis showed 2n= 36 as a normal diploid chromosome number.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleSomatic embryogenesis and ploidy stability in cassava (Manihot esculenta Crantz) cultivars regenerated by in vitro culture of young leaves.
dc.typeArtigo de periódico
dc.date.updated2018-05-15T00:33:28Zpt_BR
dc.subject.thesagroAnálise
dc.subject.thesagroCalo
dc.subject.thesagroCitogenética Vegetal
dc.subject.thesagroEmbriogénese
dc.subject.thesagroMandioca
dc.subject.thesagroManihot Esculenta
dc.subject.thesagroMicropropagação
dc.subject.thesagroVariedade
riaa.ainfo.id137733
riaa.ainfo.lastupdate2018-05-14
dc.contributor.institutionNATONIEL FRANKLIN DE MELO, CPATSA.
Aparece nas coleções:Artigo em periódico indexado (CPATSA)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Separata9620.pdf3,27 MBAdobe PDFThumbnail
Visualizar/Abrir

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace