Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/15579
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dc.contributor.authorETCHEGARAY, A.pt_BR
dc.contributor.authorBUENO, C. de C.pt_BR
dc.contributor.authorMELO, I. S. dept_BR
dc.contributor.authorTSAI, S. M.pt_BR
dc.contributor.authorFIORE, M. F.pt_BR
dc.contributor.authorSILVA-STENICO, M. E.pt_BR
dc.contributor.authorMORAES, L. A. B. dept_BR
dc.contributor.authorTESCHKE, O.pt_BR
dc.date.accessioned2016-11-22T23:00:57Z-
dc.date.available2016-11-22T23:00:57Z-
dc.date.created2008-12-22pt_BR
dc.date.issued2008pt_BR
dc.identifier.citationArchives of Microbiology, v. 190, n. 6, p.611-622, 2008.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/15579pt_BR
dc.descriptionLipopeptides produced by Bacillus subtilis are known for their high antifungal activity. The aim of this paper is to show that at high concentration they can damage the surface ultra-structure of bacterial cells. A lipopeptide extract containing iturin and surfactin (5 mg mL¡1) was prepared after isolation from B. subtilis (strain OG) by solid phase extraction. Analysis by atomic force microscope (AFM) showed that upon evaporation, lipopeptides form large aggregates (0.1?0.2 m2) on the substrates silicon and mica. When the same solution is incubated with fungi and bacteria and the system is allowed to evaporate, dramatic changes are observed on the cells. AFM micrographs show disintegration of the hyphae of Phomopsis phaseoli and the cell walls of Xanthomonas campestris and X. axonopodis. Collapses to fungal and bacterial cells may be a result of formation of pores triggered by micelles and lamellar structures, which are formed above the critical micelar concentration of lipopeptides. As observed for P. phaseoli, the process involves binding, solubilization, and formation of novel structures in which cell wall components are solubilized within lipopeptide vesicles. This is the Wrst report presenting evidences that vesicles of uncharged and negatively charged lipopeptides can alter the morphology of gramnegative bacteriapt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleEffect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-11-22T23:00:57Zpt_BR
dc.subject.thesagroFungopt_BR
dc.subject.thesagroControle biológicopt_BR
riaa.ainfo.id15579pt_BR
riaa.ainfo.lastupdate2016-11-22pt_BR
dc.contributor.institutionAugusto Etchegaray, Faculdade de Química, PUC-Campinaspt_BR
dc.contributor.institutionCarolina de Castro Bueno, Faculdade de Química, PUC-Campinaseng
dc.contributor.institutionITAMAR SOARES DE MELO, CNPMAeng
dc.contributor.institutionS. M. TSAI, CENA/USPeng
dc.contributor.institutionM. F. FIORE, CENA/USPeng
dc.contributor.institutionM. E. SILVA-STENICO, CENA/USPeng
dc.contributor.institutionL. A. B. de MORAES, Departamento de Química/USPeng
dc.contributor.institutionO. TESCHKE, Laboratório de Nanoestruturas e Interfaces, Instituto de Física, UNICAMP.eng
Aparece nas coleções:Artigo em periódico indexado (CNPMA)

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