Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/158533
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dc.contributor.authorJUST, B. J.pt_BR
dc.contributor.authorSANTOS, C. A. F.pt_BR
dc.contributor.authorFONSECA, M. E. N.pt_BR
dc.contributor.authorBOITEUX, L. S.pt_BR
dc.contributor.authorOLOIZA, B. B.pt_BR
dc.contributor.authorSIMON, P. W.pt_BR
dc.date.accessioned2011-04-09T20:30:21Z-
dc.date.available2011-04-09T20:30:21Z-
dc.date.created2007-04-20pt_BR
dc.date.issued2007pt_BR
dc.identifier.citationTheoretical and Applied Genetics, Berlin, v. 114, n. 4, p. 693-704, 2007.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/158533pt_BR
dc.descriptionCarotenoid pigments are important components of the human diet and carrots are the main dietary sources of the vitamin A precursors IX- and /3- carotene. Carotenoids play essential biological roles in plants and the genes coding for the carotenoid pathway enzymes are evolutionarily conserved, but little information exists about these genes for carrot. In this study, we utilized published carrot sequences as well as heterologous PCR approaches with primers derived from sequence information of other plant species to isolate 24 putative genes coding for carotenoid bio-synthesis enzymes in carrot. Twenty-two of these genes were placed on the carrot genetic linkage map developed from a cross between orange-rooted and white- rooted carrot. The carotenoid genes were distributed in eight of the nine linkage groups in the carrot genome recommending their use for merging maps. Two genes co-localized with a genomic region spanning one of the most significant quantitative trait loci (QTL) for carotenoid accumulation. Carotenoid biosynthesis cDNAs linked to root color mutations and to QTL for carotenoid accumulation may suggest a functional role for them as candidate genes. RACE PCR and reverse transcriptase PCR were used to amplify the full-length transcript for twenty expressed carotenoid biosynthesis genes and sequences were submitted to GenBank. The cloning and sequence information of these' genes is useful for PCR-based expression studies and may point toward transgenic approaches to manipulate carotenoid content in carrot.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMapeamentopt_BR
dc.subjectCaracterizaçãopt_BR
dc.subjectSequênciapt_BR
dc.subjectCarrotpt_BR
dc.titleCarotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-06-08T11:11:11Zpt_BR
dc.subject.thesagroCenourapt_BR
dc.subject.thesagroDaucus Carotapt_BR
dc.subject.thesagroGenomapt_BR
riaa.ainfo.id158533pt_BR
riaa.ainfo.lastupdate2018-06-08 -03:00:00pt_BR
dc.contributor.institutionCARLOS ANTONIO FERNANDES SANTOS, CPATSA.pt_BR
Appears in Collections:Artigo em periódico indexado (CPATSA)

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