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dc.contributor.authorANDRADE, A. E.
dc.contributor.authorSILVA, L. P. da
dc.contributor.authorPEREIRA, J. L.
dc.contributor.authorNORONHA, E. F.
dc.contributor.authorREIS JUNIOR, F. B. dos
dc.contributor.authorBLOCH JUNIOR, C.
dc.contributor.authorSANTOS, M. F. dos
dc.contributor.authorDOMONT, G. B.
dc.contributor.authorFRANCO, O. L.
dc.contributor.authorREIS, A. M. dos
dc.date.accessioned2018-05-22T00:37:43Z-
dc.date.available2018-05-22T00:37:43Z-
dc.date.created2008-11-15
dc.date.issued2008
dc.identifier.citationFEMS Microbiology Letters, Amsterdam, v. 281, p. 167-174, 2008.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/169382-
dc.descriptionAbstract: The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including cabbage, cauliflower and broccoli. Although the complete structural genome of X. campestris pv. campestris has been elucidated, little is known about the protein expression of this pathogen in close interaction with the host plant. Recently, a method for in vivo analysis of Xanthomonas axonopodis pv. citri was developed. In the present study, this technique was employed for the characterization of the protein expression of X. campestris pv. campestris in close interaction with the host plant Brassica oleracea. The bacterium was infiltrated into leaves of the susceptible cultivar and later recovered for proteome analysis. Recovered cells were used for protein extraction and separated by two-dimensional electrophoresis. Proteins were analysed by peptide mass fingerprinting or de novo sequencing and identified by searches in public databases. The approach used in this study may be extremely useful in further analyses in order to develop novel strategies to control this important plant pathogen.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleIn vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea.
dc.typeArtigo de periódico
dc.date.updated2018-05-22T00:37:43Zpt_BR
dc.subject.thesagroBrassica Oleracea
dc.subject.thesagroPodridão Negra
dc.subject.thesagroXanthomonas Campestris
riaa.ainfo.id169382
riaa.ainfo.lastupdate2018-05-21
dc.contributor.institutionARETUSA ELPÍDIO DE ANDRADE
dc.contributor.institutionLUCIANO PAULINO DA SILVA, CENARGENeng
dc.contributor.institutionJACKELINE L. PEREIRA, UNIVERSIDADE CATÓLICA DE BRASÍLIAeng
dc.contributor.institutionELIANE F. NORONHA, UNIVERSIDADE CATÓLICA DE BRASÍLIAeng
dc.contributor.institutionFABIO BUENO DOS REIS JUNIOR, CPACeng
dc.contributor.institutionCARLOS BLOCH JUNIOR, CENARGENeng
dc.contributor.institutionMARISE F. DOS SANTOS, UNIVERSIDADE FEDERAL DO RIO DE JANEIROeng
dc.contributor.institutionGILBERTO B. DOMONT, UNIVERSIDADE FEDERAL DO RIO DE JANEIROeng
dc.contributor.institutionOCTÁVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIAeng
dc.contributor.institutionANGELA MEHTA DOS REIS, CENARGEN.eng
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