Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/185648
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dc.contributor.authorFRANCO, M. M.eng
dc.contributor.authorANTUNES, R. C.eng
dc.contributor.authorOLIVEIRA, K. M. deeng
dc.contributor.authorPEREIRA, C. Deng
dc.contributor.authorBIASE, F. H.eng
dc.contributor.authorNUNES, F. de M. F.eng
dc.contributor.authorGOULART, L. R.eng
dc.date.accessioned2019-10-11T00:41:45Z-
dc.date.available2019-10-11T00:41:45Z-
dc.date.created2005-05-13
dc.date.issued2005
dc.identifier.other24973
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/185648-
dc.descriptionFourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and performance traits affected by GH.eng
dc.description.uribitstream/item/156101/1/a03v28n1.pdf
dc.languageeneng
dc.language.isoengeng
dc.publisherGenetics and Molecular Biology, v. 28, n. 1, p. 16-21, 2005.eng
dc.relation.ispartofEmbrapa Recursos Genéticos e Biotecnologia - Artigo em periódico indexado (ALICE)eng
dc.subjectGene PIT1eng
dc.subjectHormônio de crescimento mRNAeng
dc.subjectRT-PCR semi-quantitativoeng
dc.titleAssociation of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands.eng
dc.typeArtigo em periódico indexado (ALICE)eng
dc.date.updated2019-10-11T00:41:45Z
dc.subject.thesagroPorcoeng
dc.ainfo.id185648eng
dc.ainfo.lastupdate2019-10-10
Appears in Collections:Artigo em periódico indexado (CENARGEN)

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