Título:	Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.
Autoria:	FARIA, J. C. ALBINO, M. M. C. DIAS, B. B. A. VIANNA, M. G. R. ARAGÃO, F. J. L.
Afiliação:	JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen.
Ano de publicação:	2001
Referência:	In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTEC, 2001.
Conteúdo:	Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless.
Thesagro:	Feijão Mosaico Dourado Phaseolus Vulgaris Vírus
NAL Thesaurus:	Bean golden mosaic virus mutation
Palavras-chave:	BGMV Tolerância Biobalística Transformação
Tipo do material:	Artigo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Artigo em anais de congresso (CNPAF)