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dc.contributor.authorROMANO, E.
dc.contributor.authorSOARES, A.
dc.contributor.authorPROITE, K.
dc.contributor.authorNEIVA, S.
dc.contributor.authorGROSSI, M.
dc.contributor.authorFARIA, J. C.
dc.contributor.authorRECH, E. L.
dc.contributor.authorARAGÃO, F. J. L.
dc.date.accessioned2021-01-13T09:02:43Z-
dc.date.available2021-01-13T09:02:43Z-
dc.date.created2006-04-25
dc.date.issued2005
dc.identifier.citationGenetics and Molecular Research, v. 4, n. 2, p. 177-184, 2005.
dc.identifier.issn1676-5680
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/214084-
dc.descriptionTransgene elimination is a poorly studied phenomenon in plants. We made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. In both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. Due to its potential biotechnological value, this transgenic line was micropropagated by grafting and the vegetative copies were studied for more than two years. More than 300 plants of progeny were obtained during this period, demonstrating that the phenomenon of elimination was consistently repeated and offering an opportunity for detailed study of transgene elimination, including the characterization of the integration sites. Cloning and sequencing of the transgenic loci, reciprocal crosses to untransformed plants, genomic DNA blots, and GUS assays were performed in the transgenic lines. Based on the molecular and genetic characterization, possible mechanisms involved in transgene elimination include intrachromosomal recombination, genetic instability resulting from the tissue culture manipulations, and co-elimination of transgenes, triggered by a process of genome defense.
dc.language.isoeng
dc.rightsopenAccesseng
dc.subjectTransgene elimination
dc.titleTransgene elimination in genetically modified dry bean and soybean lines.
dc.typeArtigo de periódico
dc.subject.thesagroFeijão
dc.subject.thesagroPhaseolus Vulgaris
dc.subject.nalthesaurusbiolistics
riaa.ainfo.id214084
riaa.ainfo.lastupdate2021-01-12
dc.contributor.institutionEDUARDO ROMANO DE CAMPOS PINTO, Cenargen; ALEXANDRE SOARES; KARINA PROITE; SUZANA NEIVA; MAIRA GROSSI; JOSIAS CORREA DE FARIA, CNPAF; ELIBIO LEOPOLDO RECH FILHO, Cenargen; FRANCISCO JOSE LIMA ARAGAO, Cenargen.
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