Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/326865
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dc.contributor.authorARAÚJO, F. R.pt_BR
dc.contributor.authorCOSTA, C. M.pt_BR
dc.contributor.authorRAMOS, C. A. N.pt_BR
dc.contributor.authorFARIAS, T. A.pt_BR
dc.contributor.authorSOUZA, I. I.pt_BR
dc.contributor.authorF. dept_BR
dc.contributor.authorMELO, E. S. P.pt_BR
dc.contributor.authorELISEI, C.pt_BR
dc.contributor.authorROSINHA, G. M. S.pt_BR
dc.contributor.authorSOARES, C. O.pt_BR
dc.contributor.authorFRAGOSO, S. P.pt_BR
dc.contributor.authorFONSECA, A. H.pt_BR
dc.date.accessioned2011-07-23T01:01:25Z-
dc.date.available2011-07-23T01:01:25Z-
dc.date.created2009-02-04pt_BR
dc.date.issued2008pt_BR
dc.identifier.citationMemórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 2, p. 186-190, Mar., 2008.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/326865pt_BR
dc.descriptionAnaplasma marginale is an important vector-borne rickettsia of ruminants in tropical and subtropical regions of the world. Immunization with purified outer membranes of this organism induces protection against acute anaplasmosis. Previous studies, with proteomic and genomic approach identified 21 proteins within the outer membrane immunogen in addition to previously characterized major surface protein1a-5 (MSP1a-5). Among the newly described proteins were VirB9, VirB10, and elongation factor-Tu (EF-Tu). VirB9, VirB10 are considered part of the type IV secretion system (TFSS), which mediates secretion or cell-to-cell transfer of macromolecules, proteins, or DNA-protein complexes in Gram-negative bacteria. EF-Tu can be located in the bacterial surface, mediating bacterial attachment to host cells, or in the bacterial cytoplasm for protein synthesis. However, the roles of VirB9, VirB10, and TFSS in A. marginale have not been defined. VirB9, VirB10, and EF-Tu have not been explored as vaccine antigens. In this study, we demonstrate that sera of cattle infected with A. marginale, with homologous or heterologous isolates recognize recombinant VirB9, VirB10, and EF-Tu. IgG2 from naturally infected cattle also reacts with these proteins. Recognition of epitopes by total IgG and by IgG2 from infected cattle with A. marginale support the inclusion of these proteins in recombinant vaccines against this rickettsia.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectProteína recombinantept_BR
dc.subjectVirB9pt_BR
dc.subjectVirB10pt_BR
dc.subjectRio Grande do Nortept_BR
dc.subjectBrasilpt_BR
dc.titleIgG and IgG2 antibodies from cattle naturally infected with Anaplasma marginale recognize the recombinant vaccine candidate antigens VirB9, VirB10, and elongation factor-Tu.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-07-23T01:01:25Zpt_BR
dc.subject.thesagroAnaplasma Marginalept_BR
dc.subject.thesagroAnaplasmosept_BR
dc.subject.thesagroAnticorpopt_BR
dc.subject.thesagroImunidadept_BR
dc.subject.thesagroImunologiapt_BR
dc.subject.thesagroRuminantept_BR
dc.subject.thesagroSanidade Animalpt_BR
dc.subject.thesagroVacinapt_BR
riaa.ainfo.id326865pt_BR
riaa.ainfo.lastupdate2011-07-22pt_BR
dc.contributor.institutionFlábio Ribeiro Araújo, CNPGC; Cátia Marques da Costa, UFRRJ; Carlos Alberto do Nascimento Ramos, UFMS; Thaís Andrade Farias, bolsista CNPGC; Ingrid Ieda Fernando de Souza, , bolsista CNPGC; Elaine Silva Pádua de Melo, UFMS; Carina Elisei, bolsista CNPGC.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPGC)

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