Título:	Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep.
Autoria:	ANDREOTTI, R. MATOS, M. de F. C. GONÇALVES, K. N. OSHIRO, L. M. LIMA-JUNIOR, M. S. da C. PAIVA, F. LEITE, F. L.
Afiliação:	RENATO ANDREOTTI E SILVA, CNPGC; MARIA DE FÁTIMA CEPA MATOS, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; KELLY NODA GONÇALVES, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; LEANDRA MARLA OSHIRO, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; MANOEL SEBASTIÃO DA COSTA LIMA-JUNIOR, UFMS; FERNANDO PAIVA, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; FÁBIO LEIVAS LEITE, UNIVERSIDADE FEDERAL DE PELOTAS.
Ano de publicação:	2009
Referência:	Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 18, n.2, p. 19-22, abr.-jun, 2009.
Conteúdo:	Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep.
Thesagro:	Anticorpo Diagnostico Elisa Neospora Caninum Neosporose Ovino Parasito de Animal Sanidade Animal
Notas:	Título em português: Comparação entre ELISA baseado no antígeno recombinante NcSRS2 e RIFI para detecção de anticorpos de Neospora caninum em ovinos.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGC)