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dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.contributor.authorSTEWART, J. M.pt_BR
dc.contributor.authorOOSTERHUIS, D.pt_BR
dc.contributor.authorTURLEY, R.pt_BR
dc.contributor.authorNEUMAIER, N.pt_BR
dc.contributor.authorFARIAS, J. R. B.pt_BR
dc.date.accessioned2011-04-09T17:11:10Z-
dc.date.available2011-04-09T17:11:10Z-
dc.date.created2000-08-30pt_BR
dc.date.issued2000pt_BR
dc.identifier.citationPesquisa Agropecuaria Brasileira, Brasilia, v.35, n.7, p.1407-1416, jul. 2000.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/444817pt_BR
dc.descriptionThe aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectExpressa genicapt_BR
dc.subjectTransformacao geneticapt_BR
dc.subjectDeficit hidricopt_BR
dc.subjectBiotecnologypt_BR
dc.subjectWater deficitpt_BR
dc.subjectRNA mensageiropt_BR
dc.titleIsolation of a cotton NADP(H) oxidase homologue induced by drought stress.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.thesagroAlgodãopt_BR
dc.subject.thesagroGossypium Hirsutumpt_BR
dc.subject.thesagroBiotecnologiaeng
dc.subject.nalthesauruscottonpt_BR
dc.subject.nalthesaurusgene expressionpt_BR
dc.subject.nalthesaurusgenetic transformationpt_BR
dc.subject.nalthesaurusmessenger RNApt_BR
riaa.ainfo.id444817pt_BR
riaa.ainfo.lastupdate2004-12-09pt_BR
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