Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/46840
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dc.contributor.authorVIEIRA, E. C.
dc.contributor.authorNOGUEIRA, A. R. de A.
dc.date.accessioned2022-05-05T20:12:26Z-
dc.date.available2022-05-05T20:12:26Z-
dc.date.created2004-06-07
dc.date.issued2004
dc.identifier.citationJournal of Agricultural and Food Chemistry, v.52, p.1800-1803, 2004.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/46840-
dc.descriptionA flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectEnzymatic hysrolysis
dc.subjectImmobilization
dc.subjectPhytase
dc.subjectPhytic
dc.subjectAcid
dc.subjectSpectophometry
dc.titleOrthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.
dc.typeArtigo de periódico
riaa.ainfo.id46840
riaa.ainfo.lastupdate2022-05-05
dc.identifier.doihttps://doi.org/10.1021/jf034467f
dc.contributor.institutionEDIVAN CARVALHO VIEIRA, UFSCAR; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
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