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Campo DC | Valor | Idioma |
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dc.contributor.author | VIEIRA, E. C. | |
dc.contributor.author | NOGUEIRA, A. R. de A. | |
dc.date.accessioned | 2022-05-05T20:12:26Z | - |
dc.date.available | 2022-05-05T20:12:26Z | - |
dc.date.created | 2004-06-07 | |
dc.date.issued | 2004 | |
dc.identifier.citation | Journal of Agricultural and Food Chemistry, v.52, p.1800-1803, 2004. | |
dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/46840 | - |
dc.description | A flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively. | |
dc.language.iso | eng | |
dc.rights | openAccess | |
dc.subject | Enzymatic hysrolysis | |
dc.subject | Immobilization | |
dc.subject | Phytase | |
dc.subject | Phytic | |
dc.subject | Acid | |
dc.subject | Spectophometry | |
dc.title | Orthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis. | |
dc.type | Artigo de periódico | |
riaa.ainfo.id | 46840 | |
riaa.ainfo.lastupdate | 2022-05-05 | |
dc.identifier.doi | https://doi.org/10.1021/jf034467f | |
dc.contributor.institution | EDIVAN CARVALHO VIEIRA, UFSCAR; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. | |
Aparece nas coleções: | Artigo em periódico indexado (CPPSE)![]() ![]() |
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