Orthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.

VIEIRA, E. C.; NOGUEIRA, A. R. de A.

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DC Field	Value	Language
dc.contributor.author	VIEIRA, E. C.
dc.contributor.author	NOGUEIRA, A. R. de A.
dc.date.accessioned	2022-05-05T20:12:26Z	-
dc.date.available	2022-05-05T20:12:26Z	-
dc.date.created	2004-06-07
dc.date.issued	2004
dc.identifier.citation	Journal of Agricultural and Food Chemistry, v.52, p.1800-1803, 2004.
dc.identifier.uri	http://www.alice.cnptia.embrapa.br/alice/handle/doc/46840	-
dc.description	A flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively.
dc.language	Ingles
dc.language.iso	en
dc.rights	openAccess
dc.subject	Enzymatic hysrolysis
dc.subject	Immobilization
dc.subject	Phytase
dc.subject	Phytic
dc.subject	Acid
dc.subject	Spectophometry
dc.title	Orthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.
dc.type	Artigo de periódico
riaa.ainfo.id	46840
riaa.ainfo.lastupdate	2022-05-05
dc.identifier.doi	https://doi.org/10.1021/jf034467f
dc.contributor.institution	EDIVAN CARVALHO VIEIRA, UFSCAR; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
Appears in Collections:	Artigo em periódico indexado (CPPSE)

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