Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/471379
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dc.contributor.authorCARRARA, V. S.pt_BR
dc.contributor.authorAMATO, A. A.pt_BR
dc.contributor.authorNEVES, F. A. R.pt_BR
dc.contributor.authorBAZOTTE, R. B.pt_BR
dc.contributor.authorMANDARINO, J. M. G.pt_BR
dc.contributor.authorNAKAMURA, C. V.pt_BR
dc.contributor.authorFILHO, B. P. D.pt_BR
dc.contributor.authorCORTEZ, D. A. G.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2009-08-12pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationBrazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 42, n. 6, p. 545-550, June 2009.pt_BR
dc.identifier.issn1414-431Xpt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/471379pt_BR
dc.descriptionSince the anti-inflammatory, antidiabetic and hypolipidemic effects of soy isoflavones may be mediated by activation of peroxisome proliferator-activated receptors (PPAR), the present study investigated whether the methanolic fractions obtained from soybean seeds (E1) and soybean seed coats with hypocotyls (E2) could influence PPAR?, PPAR? and PPAR?/? transcriptional activity. The isoflavones from E1 and E2 were quantified by HPLC analysis. E1 and E2 were rich in isoflavones (daidzin, glycitin, genistin, malonyldaidzin, malonylglycitin, malonylgenistin, daidzein, glycitein, and genistein). Moreover, E1 and E2 showed no evidence of genetically modified material containing the gene CP4 EPSPS. To investigate PPAR transcriptional activity, human promonocytic U-937 cells were treated with E1 and E2 (200, 400, 800, and 1600 ?g/mL), positive controls or vehicle. Data are reported as fold-activation of the luciferase reporter driven by the PPAR-responsive element. Doseresponse analysis revealed that E1 and E2 induced the transcriptional activity of PPAR? (P < 0.001), with activation comparable to that obtained with 0.1 mM bezafibrate (positive control) at 1600 ?g/mL (4-fold) and 800 ?g/mL (9-fold), respectively. In addition, dose-response analysis revealed that E1 and E2 activated PPAR?/? (P < 0.05), and the activation at 800 ?g/mL (4- and 9-fold, respectively) was comparable to that of 0.1 mM bezafibrate (positive control). However, no effect on PPAR? was observed. Activation of PPAR? is consistent with the lipid-lowering activity of soy isoflavones in vivo, but further studies are needed to determine the physiological significance of PPAR?/? activation.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectIsoflavonapt_BR
dc.titleEffects of a methanolic fraction of soybean seeds on the transcriptional activity of peroxisome proliferator-activated receptors (PPAR).pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-07-26T11:11:11Zpt_BR
dc.subject.thesagroBioquímicapt_BR
dc.subject.thesagroNutrição Humanapt_BR
dc.subject.thesagroSojapt_BR
riaa.ainfo.id471379pt_BR
riaa.ainfo.lastupdate2017-07-26pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/S0100-879X2009000600011pt_BR
dc.contributor.institutionUEM; UnB; UnB; UEM; JOSÉ MARCOS GONTIJO MANDARINO, CNPSO; UEM; UEM; UEM.pt_BR
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