Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/488489
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorGOMES, E. A.pt_BR
dc.contributor.authorKASUYA, M. C. M.pt_BR
dc.contributor.authorBARROS, E. G. dept_BR
dc.contributor.authorBORGES, A. C.pt_BR
dc.contributor.authorARAUJO, E. F.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2004-12-20pt_BR
dc.date.issued2002pt_BR
dc.identifier.citationGenetics and Molecular Biology, Ribeirão Preto, v. 25, n. 4, p. 477-483, 2002.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/488489pt_BR
dc.descriptionInter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera and most species presented specific restriction patterns. Species not identifiable by a specific pattern belonged to two genera: Rhizopogon (R. nigrescens, R. reaii, R. roseolus, R. rubescens and Rhizopogon sp.), and Laccaria (L. bicolor and L. amethystea). Our data confirm the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEctomycorrhizal fungipt_BR
dc.subjectInternal transcribed spacer (ITS)pt_BR
dc.subjectPCR-RFLPpt_BR
dc.subjectRibosomal DNA (rDNA)pt_BR
dc.titlePolymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-05-30T11:11:11Zpt_BR
dc.subject.nalthesaurusDNA fingerprintingpt_BR
riaa.ainfo.id488489pt_BR
riaa.ainfo.lastupdate2018-05-30 -03:00:00pt_BR
dc.contributor.institutionELIANE APARECIDA GOMES, CNPMS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Polymorphisminternal.pdf1,25 MBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace