Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/488802
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dc.contributor.authorFIGUEIREDO, J. E. F.pt_BR
dc.contributor.authorGOMES, E. A.pt_BR
dc.contributor.authorGUIMARAES, C. T.pt_BR
dc.contributor.authorLANA, U. G. de P.pt_BR
dc.contributor.authorTEIXEIRA, M. A.pt_BR
dc.contributor.authorLIMA, G. V. C.pt_BR
dc.contributor.authorBRESSAN, W.pt_BR
dc.contributor.otherJOSE EDSON FONTES FIGUEIREDO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Maria Aparecida Teixeira, CNPMS; Guilherme Vitor Correa Lima, Fundação Educacional Monsenhor Messias; WELLINGTON BRESSAN, CNPMS.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2009-08-05pt_BR
dc.date.issued2009pt_BR
dc.identifier.other21696pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/488802pt_BR
dc.descriptionEndophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies.pt_BR
dc.description.uribitstream/item/31303/1/Molecular-analysis.pdfpt_BR
dc.languageenpt_BR
dc.language.isoengeng
dc.publisherBrazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009.pt_BR
dc.relation.ispartofEmbrapa Milho e Sorgo - Artigo em periódico indexado (ALICE)pt_BR
dc.subjectEndophytíc bacteriapt_BR
dc.subjectBacilluspt_BR
dc.subjectSweet cornpt_BR
dc.subjectSDS-PAGEpt_BR
dc.subjectrDNA sequencingpt_BR
dc.titleMolecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.).pt_BR
dc.typeArtigo em periódico indexado (ALICE)pt_BR
dc.date.updated2018-05-30T11:11:11Zpt_BR
dc.ainfo.id488802pt_BR
dc.ainfo.lastupdate2018-05-30 -03:00:00pt_BR
dc.identifier.doi10.1590/S1517-83822009000300014pt_BR
Appears in Collections:Artigo em periódico indexado (CNPMS)

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