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dc.contributor.authorBOMFETI, C. A.pt_BR
dc.contributor.authorSOUZA-PACCOLA, E. A.pt_BR
dc.contributor.authorMASSOLA JUNIOR, N. S.pt_BR
dc.contributor.authorMARRIEL, I. E.pt_BR
dc.contributor.authorMEIRELLES, W. F.pt_BR
dc.contributor.authorCASELA, C. R.pt_BR
dc.contributor.authorPACCOLA-MEIRELLES, L. D.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2008-06-17pt_BR
dc.date.issued2008pt_BR
dc.identifier.citationTropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/491172pt_BR
dc.descriptionThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectDoença bacteriana do milhopt_BR
dc.subjectdoenças foliares do milhopt_BR
dc.subjectMaize bacterial diseasept_BR
dc.subjectMaize leaf diseasept_BR
dc.titleLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-05-24T11:11:11Zpt_BR
dc.subject.thesagroMilhopt_BR
dc.subject.thesagroZea Mayspt_BR
riaa.ainfo.id491172pt_BR
riaa.ainfo.lastupdate2018-05-24 -03:00:00pt_BR
dc.identifier.doi10.1590/S1982-56762008000100010pt_BR
dc.contributor.institutionCleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPMS)

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