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dc.contributor.authorAARESTRUP, J. R.pt_BR
dc.contributor.authorKARAM, D.pt_BR
dc.contributor.authorFERNANDES, G. W.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2009-01-12pt_BR
dc.date.issued2008pt_BR
dc.identifier.citationGenetics and Molecular Research, Ribeirão Preto, v. 7, n. 1, p. 217-222, 2008.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/491644pt_BR
dc.descriptionEuphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome nwnber, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% co1chicine for 2 to 5 h, at ambient temperature; 0.5% co1chicine for 16 to 24 h; 0.0029 M 8-hydroxyquinoline (8-HQ) for 2 to 5 h at ambient temperature, and 0.0029 M 8-HQ for 16 to 24 h at 4 degrees C. The material was then fixed in methanol: acetic acid (3: I) and kept at -20 degrees C for 24 h. Roots were macerated in the enzyme solution ofFlaxzyme (TM) (NOVO FERMENT T)-distilled water (I: 40) at 34 degrees C for 2 h and later fixed again. Chromosome preparations were obtained by the dissociation of the apical meristems. The best chromosome preparations were obtained with the use of 8-HQ for 21 h 30 min at 4 degrees C. E. heterophylla showed 2n = 28 chromosomes. The short arm of the largest pair of chromosomes of the complement (pair nwnber 1) displayed a secondary constriction while the nuc1eolus was observed in the interphasic cell. Structural rearrangements were also observed in the E. heterophylla L. genome. The genomic instability associated with polyploidy may be the result of selection shaped by environmental adaptations and/or human-induced manipulation through agricultural practices.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPlanta daninhapt_BR
dc.titleChromosome number and cytogenetics of Euphorbia heterophylla L.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-05-24T11:11:11Zpt_BR
dc.subject.nalthesaurusevolutionpt_BR
dc.subject.nalthesauruspolyploidypt_BR
riaa.ainfo.id491644pt_BR
riaa.ainfo.lastupdate2018-05-24 -03:00:00pt_BR
dc.contributor.institutionJuliana Roriz Aarestrup, Bolsista da FAPEMIG; DECIO KARAM, CNPMS; Geraldo W. Fernandes, UFMG.pt_BR
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