Título:	Effects of two washing solutions on sperm survival of bucks.
Autor:	MACHADO, R. SIMPLICIO, A. A.
Afiliación:	RUI MACHADO, CNPC; AURINO ALVES SIMPLICIO, CNPC.
Año:	1992
Referencia:	In: INTERNATIONAL CONFERENCE ON GOATS, 5., 1992, New Delhi. Invited Papers. New Delhi: Indian Council of Agricultural Research, 1992. v. 1. p. 1089-1094.
Descripción:	The effects of 2 washing solutions on sperm survival throughout the freeze processing of buck semen were investigated. 514 ejaculates were collected with an artificial vagina from 39 mature bucks of Anglo-Nubian, Canninde, Brown alpine, Moxoto and Saanen, during 4 years and .comprising 8 year scasons (4 rainy and 4 dry). Semen was washed if it had at Icast 60% of individual! progressive motility (ipm) and 3.0 (in a scale 0 to 5) of vigour (v). The Krebs-Ringer-Phosphate solution or a half to half Krebs-Ringer-Phosphate solution plus 4.92% solution of sodium citrate (MOD solution) were used as washing solutions. The sperms were washed by two successive centrifugation at 2,400 pmr, during 15 min. All ejaculates thatshowed sediment coagulation were not frozen. The semen was thawed at 38°C during 20 sec and. 5 min after thawing the individual: progressive motility and vigour were evaluated. The sample that achieved ipm230.0% and v≥2.0 was considered useful. Efficiency of freczing (ERF) was taken between the number of useful samples divided by the total number of washed ejaculates.When MOD solution was employed no significant differences among breeds were showed (P>0.05), also there was no difference (P>0.05 between seasons, except in relation to ipm. ERF were 33.3 and 66.8% to Krebs-Ringer-Phosphate solution and MOD respectively (P<0.05). Itcan be concluded that MOD solution was more efficient than Krebs-Ringer-Phosphate solution and it allows to reduce the costs of buck semen freeze H processing.
Thesagro:	Caprino Sêmen Espermatozóide Lavagem Reprodução animal
NAL Thesaurus:	Goats Washing Spermatozoa Reproduction
Palabras clave:	Survival
Tipo de Material:	Artigo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Artigo em anais de congresso (CNPC)