Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/515320
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dc.contributor.authorALVES, F. S. F.pt_BR
dc.contributor.authorLEFEBVRE, R. B.pt_BR
dc.contributor.authorPROBERT, W.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2000-08-07pt_BR
dc.date.issued2000pt_BR
dc.identifier.citationMemórias do Instituto Oswaldo Cruz, p. 95, n. 4, p. 503-504, jul./ago. 2000.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/515320pt_BR
dc.descriptionAbstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectSequênciapt_BR
dc.subjectSequencept_BR
dc.titleAmino acid sequences of proteins from Leptospira serovar pomona.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-09-25T11:11:11Zpt_BR
dc.subject.thesagroDoença animalpt_BR
dc.subject.thesagroAminoácidopt_BR
dc.subject.thesagroProteínapt_BR
dc.subject.thesagroLeptospira pomonapt_BR
dc.subject.thesagroLeptospirosept_BR
dc.subject.nalthesaurusLeptospirapt_BR
dc.subject.nalthesaurusProteinspt_BR
dc.subject.nalthesaurusAmino acidspt_BR
dc.subject.nalthesaurusAnimal diseasespt_BR
dc.subject.nalthesaurusAntigenseng
riaa.ainfo.id515320pt_BR
riaa.ainfo.lastupdate2019-09-25 -03:00:00pt_BR
dc.contributor.institutionFRANCISCO SELMO FERNANDES ALVES, CNPCpt_BR
dc.contributor.institutionRANCE B. LEFEBVRE, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USAeng
dc.contributor.institutionWILLIAM PROBERT, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA.eng
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