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dc.contributor.authorPACHECO, L. G. C.pt_BR
dc.contributor.authorPENA, R. R.pt_BR
dc.contributor.authorCASTRO, T. L. P.pt_BR
dc.contributor.authorDORELLA, F. A.pt_BR
dc.contributor.authorBAHIA, R. C.pt_BR
dc.contributor.authorCARMINATI, R.pt_BR
dc.contributor.authorFROTA, M. N. L.pt_BR
dc.contributor.authorOLIVEIRA, S. C.pt_BR
dc.contributor.authorMEYER, R.pt_BR
dc.contributor.authorALVES, F. S. F.pt_BR
dc.contributor.authorMIYOSHI, A.pt_BR
dc.contributor.authorAZEVEDO, V.pt_BR
dc.date.accessioned2013-07-11T23:33:24Z-
dc.date.available2013-07-11T23:33:24Z-
dc.date.created2008-03-31pt_BR
dc.date.issued2007pt_BR
dc.identifier.citationJournal of Medical Microbiology, v. 56, n. 4, p. 480-486, Apr. 2007.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/533624pt_BR
dc.descriptionAbstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPCRpt_BR
dc.subjectCLApt_BR
dc.subjectBase eequenceeng
dc.subjectMolecular sequence dataeng
dc.subjectCorynebacterium Infectionseng
dc.subjectRNA bacterialeng
dc.subjectBacterial diseaseseng
dc.titleMultiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-09-26T11:11:11Zpt_BR
dc.subject.thesagroCaprinopt_BR
dc.subject.thesagroOvinopt_BR
dc.subject.thesagroCorynebacterium Pseudotuberculosispt_BR
dc.subject.thesagroLinfadenite Caseosapt_BR
dc.subject.nalthesaurusCaseous lymphadenitispt_BR
dc.subject.nalthesaurusSheep diseaseseng
dc.subject.nalthesaurusPolymerase chain reactioneng
dc.subject.nalthesaurusLymph nodeseng
riaa.ainfo.id533624pt_BR
riaa.ainfo.lastupdate2019-09-26 -03:00:00pt_BR
dc.identifier.doi10.1099/jmm.0.46997-0pt_BR
dc.contributor.institutionLUIS G. C. PACHECO, UNIVERSIDADE FEDERAL DE MINAS GERAISpt_BR
dc.contributor.institutionROBERTA R. PENA, UNIVERSIDADE FEDERAL DE MINAS GERAISeng
dc.contributor.institutionUNIVERSIDADE FEDERAL DE MINAS GERAISeng
dc.contributor.institutionUNIVERSIDADE FEDERAL DE MINAS GERAISeng
dc.contributor.institutionROBSON C. BAHIA, UNIVERSIDADE FEDERAL DA BAHIAeng
dc.contributor.institutionRENATO CARMINATI, UNIVERSIDADE FEDERAL DA BAHIAeng
dc.contributor.institutionMAURÍCIO N. L. FROTA, UNIVERSIDADE ESTADUAL VALE DO ACARAÚeng
dc.contributor.institutionSÉRGIO C. OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAISeng
dc.contributor.institutionROBERTO MEYER, UNIVERSIDADE FEDERAL DA BAHIAeng
dc.contributor.institutionFRANCISCO SELMO FERNANDES ALVES, CNPCeng
dc.contributor.institutionANDERSON MIYOSHI, UNIVERSIDADE FEDERAL DE MINAS GERAISeng
dc.contributor.institutionVASCO AZEVEDO, UNIVERSIDADE FEDERAL DE MINAS GERAIS.eng
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