Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/543166
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Campo DCValorIdioma
dc.contributor.authorBERENSCHOT, A. S.
dc.contributor.authorZUCCHI, M. I.
dc.contributor.authorTULMANN-NETO, A.
dc.contributor.authorQUECINI, V.
dc.date.accessioned2026-06-15T22:33:54Z-
dc.date.available2026-06-15T22:33:54Z-
dc.date.created2008-12-12
dc.date.issued2008
dc.identifier.citationBrazilian Journal of Plant Physiology, Pelotas, v. 20, n. 2, p. 95-103, 2008.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/543166-
dc.descriptionTraditionally, mutagenesis has been used to introduce novel genetic variability in ornamental crops. More recently, it has become a powerful tool in gene discovery and functional analyses in reverse genetics approaches. The present work aimed to compare the efficiency of physical and chemical agents in generating mutant populations of petunia. We have indirectly evaluated the genomic damage by analyzing developmental characteristics of the plantlets derived from treated seeds employing gamma radiation at 0, 20, 40, 60, 80 and 100 Gy and the alkylating agent ethyl-methanesulfonate (EMS) at 0, 0.05, 0.1, 0.15, 0.2 and 0.25% (v/v). Gamma rays and EMS caused developmental defects and decreased seedling viability in plants obtained from the mutagenized seeds. High mutagen doses reduced in approximately 44% the number of plants with primary leaves at 15 days after sowing (DAS) and decreased seedling survival rates to 55% (gamma) and 28% (EMS), in comparison to untreated controls. Seedling height decrease was proportional to increasing EMS dosage, whereas 40 and 60 Gy of gamma irradiation caused the most significant reduction in height. Moderate DNA damage allowing a high saturation of mutant alleles in the genome and the generation of viable plants for reverse genetics studies was correlated to the biological parameter LD50 , the dose required to kill half of the tested population. It corresponded to 100 Gy for gamma radiation and 0.1% for EMS treatment. The optimized mutagen treatments were used to develop petunia mutant populations (M1 and M2 ) and novel morphological mutants were identified.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectMorfologia
dc.subjectMutagênese
dc.subjectradiação gama
dc.subjectDano genômico
dc.titleMutagenesis in Petunia x Hybrida Vilm. and isolation of a novel morphological mutant.
dc.typeArtigo de periódico
dc.subject.thesagroBiologia
dc.subject.thesagroGenética
dc.subject.nalthesaurusPetunia
dc.description.notesTítulo em português: Mutagênese em Petunia x hybrida Vilm. e isolamento de um novo mutante morfológico.
riaa.ainfo.id543166
riaa.ainfo.lastupdate2026-06-15
dc.contributor.institutionAMANDA S. BERENSCHOT, INSTITUTO AGRONÔMICO; MARIA I. ZUCCHI, INSTITUTO AGRONÔMICO; AUGUSTO TULMANN-NETO, UNIVERSIDADE DE SÃO PAULO; VERA MARIA QUECINI, CNPUV.
Aparece nas coleções:Artigo em periódico indexado (CNPUV)

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