Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/577024
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dc.contributor.authorJONSSON, C. M.pt_BR
dc.contributor.authorAOYAMA, H.pt_BR
dc.date.accessioned2016-06-04T09:06:45Z-
dc.date.available2016-06-04T09:06:45Z-
dc.date.created2009-12-02pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationBiometals, v. 23, n. 1, p. 93-98, Sep. 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/577024pt_BR
dc.descriptionThe in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectEcotoxicologiapt_BR
dc.titleEffect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-06-04T09:06:45Zpt_BR
riaa.ainfo.id577024pt_BR
riaa.ainfo.lastupdate2016-06-03pt_BR
dc.identifier.doi10.1007/s10534-009-9270-zpt_BR
dc.contributor.institutionCLAUDIO MARTIN JONSSON, CNPMA; HIROSHI AOYAMA, IB-UNICAMP.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPMA)

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