Detection of human interchromosomal trans-splicing in sequence databanks.

HERAI, R. H.; YAMAGISHI, M. E. B.

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Campo DC	Valor	Idioma
dc.contributor.author	HERAI, R. H.	pt_BR
dc.contributor.author	YAMAGISHI, M. E. B.	pt_BR
dc.date.accessioned	2011-04-10T11:11:11Z	pt_BR
dc.date.available	2011-04-10T11:11:11Z	pt_BR
dc.date.created	2009-12-15	pt_BR
dc.date.issued	2010	pt_BR
dc.identifier.citation	Briefings in Bioinformatics, London, v. 2, n. 2, p. 198-209, 2010.	pt_BR
dc.identifier.uri	http://www.alice.cnptia.embrapa.br/alice/handle/doc/577947	pt_BR
dc.description	Trans-splicing is a common phenomenon in nematodes and kinetoplastids, and it has also been reported in other organisms, including humans. Up to now, all in silico strategies to find evidence of trans-splicing in humans have required that the candidate sequences follow the consensus splicing site rules (spliceosome-mediated mechanism). However, this criterion is not supported by the best human experimental evidence, which, except in a single case, do not follow canonical splicing sites. Moreover, recent findings describe a novel alternative tRNA mediated trans-splicing mechanism, which prescinds the spliceosome machinery. In order to answer the question, ?Are there hybrid mRNAs in sequence databanks, whose characteristics resemble those of the best human experimental evidence??, we have developed a methodology that successfully identified 16 hybrid mRNAs which might be instances of interchromosomal trans-splicing. Each hybrid mRNA is formed by a trans-spliced region (TSR), which was successfully mapped either onto known genes or onto a human endogenous retrovirus (HERV-K) transcript which supports their transcription. The existence of these hybrid mRNAs indicates that trans-splicing may be more widespread than believed. Furthermore, non-canonical splice site patterns suggest that infrequent splicing sites may occur under special conditions, or that an alternative trans-splicing mechanism is involved. Finally, our candidates are supposedly from normal tissue, and a recent study has reported that trans-splicing may occur not only in malignant tissues, but in normal tissues as well. Our methodology can be applied to 5'-UTR, coding sequences and 3'-UTR in order to find new candidates for a posteriori experimental confirmation.	pt_BR
dc.language.iso	eng	eng
dc.rights	openAccess	eng
dc.subject	Bioinformática	pt_BR
dc.subject	Inter-chromosomal trans-splicing	pt_BR
dc.subject	Non-canonical splicing sites	pt_BR
dc.subject	TRNA-mediatedtrans-splicing	pt_BR
dc.subject	Banco de dados	pt_BR
dc.title	Detection of human interchromosomal trans-splicing in sequence databanks.	pt_BR
dc.type	Artigo de periódico	pt_BR
dc.date.updated	2016-10-13T11:11:11Z	pt_BR
dc.subject.nalthesaurus	Bioinformatics	pt_BR
riaa.ainfo.id	577947	pt_BR
riaa.ainfo.lastupdate	2016-10-13	pt_BR
dc.identifier.doi	10.1093/bib/bbp041	pt_BR
dc.contributor.institution	ROBERTO HIROCHI HERAI, CNPTIA; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA.	pt_BR
Aparece nas coleções:	Artigo em periódico indexado (CNPTIA)

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