Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/580267
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dc.contributor.authorMARTINS, F. A.pt_BR
dc.contributor.authorCARNEIRO, P. C. S.pt_BR
dc.contributor.authorCRUZ, C. D.pt_BR
dc.contributor.authorCARNEIRO, J. E. de S.pt_BR
dc.contributor.authorGUIMARAES, C. T.pt_BR
dc.date.accessioned2017-06-09T23:55:26Z-
dc.date.available2017-06-09T23:55:26Z-
dc.date.created2010-01-13pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationPesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1291-1296, out. 2009.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/580267pt_BR
dc.descriptionThe objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectOrigem alélicapt_BR
dc.subjectHeterozigotopt_BR
dc.subjectPopulações naturaispt_BR
dc.subjectPoligenespt_BR
dc.subjectPCR semiquantitativapt_BR
dc.titleEndosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2018-05-25T11:11:11Zpt_BR
dc.subject.thesagroZea Mayspt_BR
riaa.ainfo.id580267pt_BR
riaa.ainfo.lastupdate2018-05-25 -03:00:00pt_BR
dc.identifier.doi10.1590/S0100-204X2009001000012pt_BR
dc.contributor.institutionFRANCIELLE ALLINE MARTINS, UFV; PEDRO CRESCÊNCIO SOUZA CARNEIRO, UFV; COSME DAMIÃO CRUZ, UFV; JOSÉ EUSTÁQUIO DE SOUZA CARNEIRO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPMS)

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