Título:	Retail survey of Brazilian milk and minas frescal cheese and a contaminated dairy plant to establish prevalence, relatedness, and sources of listeria monocytogenes isolates.
Autoria:	BRITO, J. R. F. SANTOS, E. M. P. ARCURI, E. F. LANGE, C. C. BRITO, M. A. V. P. SOUZA, G. N. CERQUEIRA, M. M. O. P. BELTRAN, J. M. S. CALL, J. E. LIU, Y. PORTO-FETT, A. C. S. LUCHANSKY, J. B.
Afiliação:	José Renaldi Feitosa Brito, Embrapa Gado de Leite; Emília Maricato Pedro dos Santos, UFMG; Edna Froeder Arcuri, Embrapa Gado de Leite; Carla Christine Lange, Embrapa Gado de Leite; Maria Aparecida Vasconcelos Paiva e Brito, Embrapa Gado de Leite; Guilherme Nunes de Souza, Embrapa Gado de Leite; Mônica Maria Oliveira Pinho Cerqueira, UFMG; J. Marcela Soto Beltran Centro de Investigación en Alimentación y Desarrolo Unidad Culiacán Mexico; Jeffrey E. Call U.S. Department of Agriculture ARS Eastern Regional Research Center MFSRU Wyndmoor Pennsylvania; Yanhong Liu U.S. Department of Agriculture ARS Eastern Regional Research Center MFSRU Wyndmoor Pennsylvania; Anna C. S. Porto-Fett U.S. Department of Agriculture ARS Eastern Regional Research Center MFSRU Wyndmoor Pennsylvania; John B. Luchansky U.S. Department of Agriculture ARS Eastern Regional Research Center MFSRU Wyndmoor Pennsylvania.
Ano de publicação:	2008
Referência:	Applied and Environmental Microbiology, v. 74, n. 15, p. 4954-4961, 2008.
Conteúdo:	A study was designed to recover Listeria monocytogenes from pasteurized milk and Minas frescal cheese (MFC) sampled at retail establishments (REs) and to identify the contamination source(s) of these products in the corresponding dairy processing plant. Fifty milk samples (9 brands) and 55 MFC samples (10 brands) were tested from REs located in Juiz de Fora, Minas Gerais, Brazil. All milk samples and 45 samples from 9 of 10 MFC brands tested negative for L. monocytogenes; however, “brand F” of MFC obtained from REs 119 and 159 tested positive. Thus, the farm/plant that produced brand F MFC was sampled; all samples from the milking parlor tested negative for L. monocytogenes, whereas several sites within the processing plant and the MFC samples tested positive. All 344 isolates recovered from retail MFC, plant F MFC, and plant F environmental samples were serotype 1/2a and displayed the same AscI or ApaI fingerprints. Since these results established that the storage coolers served as the contamination source of the MFC, plant F was closed so that corrective renovations could be made. Following renovation, samples from sites that previously tested positive for the pathogen were collected from the processing environment and from MFC on multiple visits; all tested negative for L. monocytogenes. In addition, on subsequent visits to REs 159 and 119, all MFC samples tested negative for the pathogen. Studies are ongoing to quantify the prevalence, levels, and types of L. monocytogenes in MFC and associated processing plants to lessen the likelihood of listeriosis in Brazil.
NAL Thesaurus:	pasteurized milk
Palavras-chave:	Minas frescal cheese Contamination sources
Digital Object Identifier:	https://doi.org/10.1128/AEM.01828-07
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGL)