Título:	A simple chromosomal marker can reliably distinguishes Poncirus from Citrus species.
Autoria:	BRASILEIRO-VIDAL, A. C. SEREJO, J. A. dos S. SOARES FILHO, W. dos S. GUERRA, M.
Afiliação:	A. C. BRASILEIRO-VIDAL, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; WALTER DOS SANTOS SOARES FILHO, CNPMF; M. GUERRA, UNIVERSIDADE FEDERAL DE PERNAMBUCO.
Ano de publicação:	2007
Referência:	Genetica, Netherlands, v. 129, n. 3, p. 273-279, mar. 2007.
Conteúdo:	Abstract: Several chromosome types have been recognized in Citrus and related genera by chromomycin A(3 )(CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata x C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA(+) banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus x Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm.
Thesagro:	Fruta Cítrica Cromossoma Marcador Genético Marcador Molecular Retrocruzamento Cariótipo Hibrido Germoplasma Laranja
NAL Thesaurus:	Citrus fruits Chromosomes Genetic markers Molecular genetics Backcrossing Hybrids Germplasm Oranges
Palavras-chave:	Chromosome CMA rDNA
Digital Object Identifier:	DOI 10.1007/s10709-006-0007-4
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPMF)