Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/660121
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dc.contributor.authorFUGANTI, R.pt_BR
dc.contributor.authorMACHADO, M. de F. P. da S.pt_BR
dc.contributor.authorLOPES, V. S.pt_BR
dc.contributor.authorSILVA, J. F. V.pt_BR
dc.contributor.authorARIAS, C. A. A.pt_BR
dc.contributor.authorMARIN, S. R. R.pt_BR
dc.contributor.authorBINNECK, E.pt_BR
dc.contributor.authorABDELNOOR, R. V.pt_BR
dc.contributor.authorMARCELINO, F. C.pt_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2010-03-08pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationJournal of Biomedicine and Biotechnology, 2010.pt_BR
dc.identifier.issn1110-7251pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/660121pt_BR
dc.descriptionDuring earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMarcadores molecularespt_BR
dc.subjectSoybeanpt_BR
dc.subjectNematóide de galhapt_BR
dc.titleSize of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-08-03T11:11:11Zpt_BR
dc.subject.thesagroMeloidogyne Javanicapt_BR
dc.subject.thesagroNematóidept_BR
dc.subject.thesagroSojapt_BR
dc.format.extent29 p.pt_BR
riaa.ainfo.id660121pt_BR
riaa.ainfo.lastupdate2017-08-03pt_BR
dc.identifier.doi10.1155/2010/847673pt_BR
dc.contributor.institutionRENATA FUGANTI, UEM; MARIA DE FÁTIMA PIRES DA SILVA MACHADO, UEM; VALÉRIA STEFANIA LOPES, UNIFIL; JOAO FLAVIO VELOSO SILVA, CPAMT; CARLOS ALBERTO ARRABAL ARIAS, CNPSo; SILVANA REGINA ROCKENBACH MARIN, CNPSo; ELISEU BINNECK, CNPSo; RICARDO VILELA ABDELNOOR, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSo; ALEXANDRE LIMA NEPOMUCENO, CNPSo.pt_BR
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