Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/697142
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dc.contributor.authorMATALLO, M. B.pt_BR
dc.contributor.authorALMEIDA, S. D. B.pt_BR
dc.contributor.authorCERDEIRA, A. L.pt_BR
dc.contributor.authorFRANCO, D. A. S.pt_BR
dc.contributor.authorBLANCO, F. M. G.pt_BR
dc.contributor.authorMENEZES, P. T. C.pt_BR
dc.contributor.authorLUCHINI, L. C.pt_BR
dc.contributor.authorMOURA, M. A.pt_BR
dc.contributor.authorDUKE, S. O.pt_BR
dc.date.accessioned2016-06-04T09:07:51Z-
dc.date.available2016-06-04T09:07:51Z-
dc.date.created2010-04-20pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationPlanta Daninha, Viçosa, v. 27, p. 987-994, 2009. Número Especial.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/697142pt_BR
dc.descriptionA better method for determination of shikimate in plant tissues is needed to monitor exposure of plants to the herbicide glyphosate [N-(phosphonomethyl)glycine] and to screen the plant kingdom for high levels of this valuable phytochemical precursor to the pharmaceutical oseltamivir. A simple, rapid, and efficient method using microwave-assisted extraction (MWAE) with water as the extraction solvent was developed for the determination of shikimic acid in plant tissues. High performance liquid chromatography was used for the separation of shikimic acid, and chromatographic data were acquired using photodiode array detection. This MWAE technique was successful in recovering shikimic acid from a series of fortified plant tissues at more than 90% efficiency with an interference-free chromatogram. This allowed the use of lower amounts of reagents and organic solvents, reducing the use of toxic and/or hazardous chemicals, as compared to currently used methodologies. The method was used to determine the level of endogenous shikimic acid in several species of Brachiaria and sugarcane (Saccharum officinarum) and on B. decumbens and soybean (Glycine max) after treatment with glyphosate. The method was sensitive, rapid and reliable in all cases.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGlifosatopt_BR
dc.titleMicrowave-assisted solvent extraction and analysis of shikimic acid from plant tissues.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-06-04T09:07:51Zpt_BR
riaa.ainfo.id697142pt_BR
riaa.ainfo.lastupdate2016-06-03pt_BR
dc.contributor.institutionM. B. MATALLO, Weed Science Laboratory, Biological Institute, IBpt_BR
dc.contributor.institutionS. D. B. ALMEIDA, Weed Science Laboratory, Biological Institute, IBeng
dc.contributor.institutionANTONIO LUIZ CERDEIRA, CNPMAeng
dc.contributor.institutionD. A. S. FRANCO, Weed Science Laboratory, Biological Institute, IBeng
dc.contributor.institutionF. M. G. BLANCO, Weed Science Laboratory, Biological Institute, IBeng
dc.contributor.institutionP. T. C. MENEZES, Weed Science Laboratory, Biological Institute, IBeng
dc.contributor.institutionL. C. LUCHINI, Agrochemicals Ecotoxicology Laboratory, Biological Institute, IBeng
dc.contributor.institutionM. A. MOURA, Weed Science Laboratory, Biological Institute, IBeng
dc.contributor.institutionS. O. DUKE, Natural Products Utilization Research Unit, USDA, ARS.eng
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