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dc.contributor.authorASSIS, C. R. D.pt_BR
dc.contributor.authorAMARAL, I. P. G.pt_BR
dc.contributor.authorCASTRO, P. F.pt_BR
dc.contributor.authorCARVALHO JÚNIOR, L. B.pt_BR
dc.contributor.authorBEZERRA, R. S.pt_BR
dc.date.accessioned2011-04-09T18:58:58Z-
dc.date.available2011-04-09T18:58:58Z-
dc.date.created2008-03-22pt_BR
dc.date.issued2007pt_BR
dc.identifier.citationEnvironmental Toxicology and Chemistry, v. 26, n. 7, p. 1451-1453, 2007.pt_BR
dc.identifier.issn0730-7268pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/69727pt_BR
dc.descriptionDichlorvos is an acutely toxic organophosphorous pesticide that is known as a classical acetylcholinesterase (AChE; EC 3.1.1.7) inhibitor. Here, the brain AChE from the important Amazonian fish tambaqui (Colossoma macropomum) was assayed in the presence of this insecticide and also of deltamethrin, a classical sodium and potassium channel inhibitor (negative control). Four tissue homogenates were analyzed in triplicate for AChE activity using acetylthiocholine as the substrate and 5,5'-dithiobis(2-nitrobenzoic) acid (DTNB) as the color-developing agent. Each tissue homogenate represented pooled brains from five fish. The inhibitory effect of dichlorvos on AChE activities was determined at concentrations from 0.001 to 10 ppm and compared to controls. This effect followed an exponential decay model (y = 9.420 + 26.192e(-x/5.380); r2 = 0.989), presenting IC50 (the concentration of dichlorvos that is required for 50% of AChE inhibition) of 0.081 ppm (0.368 umol/L). No effect was observed for the deltamethrin, and the concentration 0.0452 umol/L of dichlorvos was significantly different from this control. These results suggest that tambaqui brain AChE can be proposed as a biomarker for dichlorvos and can be used as a tool for aquatic environment monitoring.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectOrganophosphorous pesticidept_BR
dc.subjectBiomarkerpt_BR
dc.titleEffect of Dichlorvos on the acetylcholinesterase from tambaqui (Colossoma macropomum) Brain.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.thesagroColossoma Macropomumpt_BR
dc.subject.thesagroTambaquipt_BR
dc.subject.nalthesaurusacetylcholinesterasept_BR
dc.subject.nalthesaurusdichlorvospt_BR
riaa.ainfo.id69727pt_BR
riaa.ainfo.lastupdate2010-12-29pt_BR
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