Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/697368
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dc.contributor.authorFONSECA, I.pt_BR
dc.contributor.authorSILVA, P. V.pt_BR
dc.contributor.authorLANGE, C. C.pt_BR
dc.contributor.authorGUIMARAES, M. F. M.pt_BR
dc.contributor.authorWELLER, M. M. D. C. A.pt_BR
dc.contributor.authorSOUSA, K. R. S.pt_BR
dc.contributor.authorLOPES, P. S.pt_BR
dc.contributor.authorGUIMARÃES, J. D.pt_BR
dc.contributor.authorGUIMARÃES, S. E. F.pt_BR
dc.date.accessioned2011-04-09T16:08:46Z-
dc.date.available2011-04-09T16:08:46Z-
dc.date.created2010-04-22pt_BR
dc.date.issued2009pt_BR
dc.identifier.citationGenetics and Molecular Biology, Ribeirão Preto, v. 32, n. 4, p. 776-781, 2009.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/697368pt_BR
dc.descriptionIn order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectCytokinept_BR
dc.subjectReal-time PCRpt_BR
dc.titleExpression profile of genes associated with mastitis in dairy cattle.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.nalthesaurusgene expressionpt_BR
dc.subject.nalthesaurusimmune responsept_BR
riaa.ainfo.id697368pt_BR
riaa.ainfo.lastupdate2010-12-07pt_BR
dc.identifier.doihttps://doi.org/10.1590/S1415-47572009005000074eng
dc.contributor.institutionISABELA FONSECA, UFV; PRISCILA VENDRAMINI SILVA, UFV; CARLA CHRISTINE LANGE, CNPGL; MARTA FONSECA MARTINS GUIMARAES, CNPGL; MAYARA MORENA DEL CAMBRE AMARAL WELLER, UFV; KATIENE RÉGIA SILVA SOUSA, UFV; PAULO SÁVIO LOPES, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE E. F. GUIMARÃES, UFV.pt_BR
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